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Plasmid#176740PurposeExpresses the promiscuous small biotin ligase, BioID2, in astrocytesDepositorInsertBioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pLenti PGK Blast DEST (w524-1)
Plasmid#19065Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, BlastÂDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
GPR173-Tango
Plasmid#66338PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR173 (GPR173 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 huZAP(L)
Plasmid#45907DepositorInsertZAP(L) isoform (ZC3HAV1 Human)
UseTagsHAExpressionMammalianMutationM202T, L388F, Q701E, T851I (reference NP_064504.2)PromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1wt-C1
Plasmid#131818PurposeExpresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsHcRedExpressionMammalianMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
M7 pdnPKA-GFP
Plasmid#16716DepositorInsertdnPKA (Prkar1a Mouse)
UseXenopus expressionTagsGFPExpressionMutationDominant negative. Mutations at cAMP binding site…PromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
TFORF0263
Plasmid#142500PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-dCas13-APO1-YTH
Plasmid#204767PurposeGEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)DepositorInsertdCas13-NLS-APO1-YTH-HA
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEf1alphaAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA AKT1
Plasmid#78778PurposeTo overexpress AKT1 in Mammalian CellsDepositorInsertAKT1 (AKT1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pbabe-c-mycT58A+HRasG12V
Plasmid#11130DepositorUseRetroviralTagsExpressionMammalianMutationc-myc T58A, A156T, and G438R; and H-Ras G12VPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMV306hsp+FFluc
Plasmid#26156DepositorInsertFirefly luciferase
UseTagsExpressionBacterialMutationFirefly luciferase gene codon optimised for mycob…PromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-GFP-Tmem192
Plasmid#134630Purposelysosomal markerDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTagsFLAG and GFPExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Hygro DEST ERKKTRmCerulean3
Plasmid#90229PurposeLentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
Plasmid#158967PurposeUbiquitous expression of mtON in C. elegansDepositorInserteft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
UseTagsGFPExpressionWormMutationPromotereft-3Available sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE Hygro Flag hTERT
Plasmid#53546Purposeretroviral expression of human TERTDepositorInsertTERT (TERT Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI-VSVG
Plasmid#1733PurposeVSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan labDepositorInsertVSV-G Envelope
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMal-E6LLO
Plasmid#51281PurposeExpresses a fibronectin fusion with listeriolysin targeting EGFR in bacteriaDepositorInsertE6LLO
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
sgOpti
Plasmid#85681PurposeLentiviral vector to express sgRNAs. The cloning site is BsmBI.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpdCas9 VPR
Plasmid#188509PurposeExpresses FLAG tagged SpdCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/H840APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only