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Showing: 10721 - 10740 of 52188 results

  1. thoc5.018.389

    Plasmid
    #137266
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    thoc5 (THOC5 Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    018-389
    Promoter
    Available since
    Feb. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    137266-plasmid-map-sequence-id-323966

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  2. NKAP.001.140

    Plasmid
    #137206
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    NKAP (NKAP Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-140
    Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    137206-plasmid-map-sequence-id-322594

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  3. Nup54-mEGFP494(2)

    Plasmid
    #163428
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163428-plasmid-map-sequence-id-321665

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  4. pGEX4-4T-1 Pontibacillus AGSC

    Plasmid
    #161814
    Purpose
    Expresses AGSC domain from Pontibacillus A/G cyclase enzyme
    Depositor
    Douglas Feldman
    Insert
    AGSC domain
    Use
    Tags
    His and TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161814-plasmid-map-sequence-id-319428

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  5. pLIX403-EBFP2-ccdB-EYFP

    Plasmid
    #158558
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    158558-plasmid-map-sequence-id-309874

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  6. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

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  7. YARS2_pNIC-CTB10H

    Plasmid
    #153060
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase 2
    Depositor
    Susanne Gräslund
    Insert
    YARS2 (YARS2 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153060-plasmid-map-sequence-id-304480

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  8. UC16m

    Plasmid
    #121038
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gQi Cas9 gRNA UC part
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121038-plasmid-map-sequence-id-236383

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  9. UC17m

    Plasmid
    #121039
    Purpose
    MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gV1 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121039-plasmid-map-sequence-id-236352

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  10. UCT1m

    Plasmid
    #121041
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UCT-part guide RNA (Stanley Qi sequence)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121041-plasmid-map-sequence-id-245418

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  11. C108m

    Plasmid
    #121010
    Purpose
    MoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding mScarlet variant, codon-optimized for E. col, with pdt3 degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121010-plasmid-map-sequence-id-236173

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  12. C114m

    Plasmid
    #121013
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9 with no bbsI or BsaI sites
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121013-plasmid-map-sequence-id-245424

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  13. C103m

    Plasmid
    #121007
    Purpose
    MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121007-plasmid-map-sequence-id-236162

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  14. 6NEY

    Plasmid
    #127295
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD3
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    127295-plasmid-map-sequence-id-247860

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  15. 6MF9

    Plasmid
    #127290
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD12
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127290-plasmid-map-sequence-id-247040

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  16. pAS1-rab-3P::his-24::mOrange

    Plasmid
    #124346
    Purpose
    Nuclear Localized Pan-Neuronal mOrange
    Depositor
    Andrew Leifer
    Insert
    rab-3, mOrange (rab-3 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124346-plasmid-map-sequence-id-241953

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  17. pAS1-lim-4P::his-24::Cerulean

    Plasmid
    #124336
    Purpose
    Multicolor Worms for Neuronal ID
    Depositor
    Andrew Leifer
    Insert
    lim-4, Cerulean (lim-4 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124336-plasmid-map-sequence-id-241923

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  18. MoClo adapted pUC57-Amp Level 1 position 2

    Plasmid
    #109221
    Purpose
    Non binary level 1 position 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants
    Depositor
    Elena Caro Bernat
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    109221-plasmid-map-sequence-id-234968

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  19. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Christopher Reisch
    Insert
    SpCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120227-plasmid-map-sequence-id-233288

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  20. pJR288

    Plasmid
    #78550
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.
    Depositor
    John Rossi
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    78550-plasmid-map-sequence-id-191096

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Showing: 10721 - 10740 of 52188 results