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Plasmid#39348DepositorUseTags3xFLAG, 6xHis, and human FcExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only
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pMuLE Lenti Dest eGFP
Plasmid#62175PurposeMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseLentiviral; Mule gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
MuHKII-pGFPN3
Plasmid#21922DepositorInsertMutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK2 Human)
UseTagsGFPExpressionMammalianMutationThis is the full length human HKII cDNA sequence …PromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody
Plasmid#138132PurposeExpresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertEGFP-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
5HT6-G-GECO1.0
Plasmid#47499PurposeFluorescent reporter for Ca2+ inside primary ciliaDepositorInsert5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
UseTagsG-GECO1.0ExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMCS.DT-A
Plasmid#22730DepositorInsertDT-A
UseBac recombineeringTagsExpressionMutationThis is a gap-repair vector designed for retrievi…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
TrHKII-pGFPN3
Plasmid#21921DepositorInsertTruncated human HKII cDNA (lacking the sequence encoded by exon 1, which is the mitochondrial binding domain) in pGFP-N3 plasmid (HK2 Human)
UseTagsGFPExpressionMammalianMutationThis is the truncated human HKII cDNA sequence (l…PromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SIRT6_H133Y
Plasmid#53149Purposeexpression vector for catalyitc inactive human Sirtuin6DepositorInsertSirt6 (SIRT6 Human)
UseTagsFLAGExpressionMammalianMutationH133Y, catalytic inactivePromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS
Plasmid#199355PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFA-SUMOstarExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A hu_ncOGT WT (open N-term)
Plasmid#155197PurposeEntry vector encoding non-tagged O-GlcNAc Transferase with open N-terminusDepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway cloningTagsExpressionMutationWT OGT with no start codon, in-frame with gateway…PromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
IDE-2Strep
Plasmid#210355PurposeExpresses IDE fused with 2Strep in mammalian cellsDepositorInsertIDE (IDE Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Non-specific sgRNA
Plasmid#109432PurposeMLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.DepositorInsertNon-specific gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT PUR mGSK3A K148A
Plasmid#35158DepositorInsertGSK-3α (Gsk3a Mouse)
UseTagsExpressionMammalianMutationK148A (dominant negative)PromoterCMVAvailable sinceMay 18, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)
Plasmid#139446PurposeLentiviral vector genome to ectopically express Firefly luciferaseDepositorInsertFLAG-tag Firefly luciferase
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX6-PX (27-171)
Plasmid#119087PurposeBacterial expression of human phox homology (PX) domain, SNX6-PX (27-171)DepositorInsertSNX6-PX (29-170) (SNX6 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
NSP3C-Flag
Plasmid#210349PurposeExpresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cellsDepositorInsertC terminal of SARS-CoV-2 NSP3 (ORF1ab )
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (1-249)
Plasmid#42204DepositorInsertc-SRC (1-249) (SRC Human)
UseTagsHAExpressionMammalianMutationcontains amino acids 1-249PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 Y42A/M43A
Plasmid#19940DepositorInsertcullin 1 Y42A/M43A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationCUL1 with a SKP1 binding mutantPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
mCherry Codon 59 sgRNA
Plasmid#109431PurposeMLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporterDepositorInsertmCherry codon 59 gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-FIH1
Plasmid#21403DepositorInsertHIF1AN (HIF1AN Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only