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Plasmid#168462PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only
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FUW-ubiquitin-EphB4-SV40-GFP (B4G)
Plasmid#65444PurposeExpression of human EPHB4 and GFP in mammalian cellsDepositorInsertEPHB4 (EPHB4 Human)
UseLentiviralTagsSV40-EGFPExpressionMammalianMutationPromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC4A5
Plasmid#132284PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC4A5 (SLC4A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF-DEST51-GOLPH3
Plasmid#21690DepositorInsertGolgi Phosphoprotein 3 (GOLPH3 Human)
UseInvitrogen gateway destination vectorTags6xHIS and V5ExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-ETV1
Plasmid#98623PurposeGateway entry TOPO TA cloning vector for ETV1DepositorInsertETV1 (Etv1 Mouse)
UseTopo ta cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA
Plasmid#134925PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.DepositorInsertCascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262B-ABE8e
Plasmid#161524PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editingDepositorInsertTadA8e-zSpRYCas9(D10A)
UseCRISPR; Gateway compatible tada8e-zsprycas9(d10a)…TagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-GFP-TRIM28
Plasmid#185010PurposeEncodes gRNA for mouse TRIM28 along with Cas9 with 2A-EGFPDepositorInsertTRIM28 sgRNA (Trim28 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv9_CBX7_Chromo/TAF3.PHD-13XL-BASU
Plasmid#179415PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.Chromo/TAF3.PHD-13XL-BASUDepositorInsertCBX7.Chromo/TAF3.PHD-13XL-BASU
UseTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT D554N (Puro)
Plasmid#154292PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationD554N mutation (Ser/Thr glycosylation-incompetent)PromoterPGKAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-pdh_RiboJ_mCherry_Bba_B0015
Plasmid#107581PurposeB. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpdh promoter Bacillus megaterium DSM319Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3EF5a-PIK3CA H1047R
Plasmid#180034PurposeEntry vector of PIK3CA H1047R for gateway cloning.DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseEntry vectorTagsHA tagExpressionMammalianMutationH1047RPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 WT
Plasmid#74950Purposemammalian expression of Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
t1-435
Plasmid#166578PurposeFor expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertTln1 Head (1-435) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-435 onlyPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf1(WT)-mCherry
Plasmid#79419PurposeExpresses C-terminally mCherry-tagged Arf1(WT) in mammalian cellsDepositorInsertArf1 (ARF1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC26A7
Plasmid#132152PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC26A7 (SLC26A7 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti_EF1a_Flag_TP53_P278A
Plasmid#183113PurposeExpresses flag-tagged p53 with P278A mutation in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged proline 278 to alanine, see depositor com…PromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PSAT
Plasmid#74115PurposeExpression of PSATDepositorInsertpET28a-PSAT (PSAT1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKII-GFP-P2A-bReaches-minWPRE
Plasmid#118276PurposeExpresses EGFP and bReaChES under a minimal CamKII PromoterDepositorInsertsEGFP
bReaCHES
UseAAVTagsP2AExpressionMutationPromoterCamK IIAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCVhyg-HA-FLAG_Ubc9_WT
Plasmid#164936PurposeExpresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNADepositorInserthuman UBC9 (UBE2I Human)
UseRetroviralTagsFLAG & HA tagsExpressionMutationPromoterPGK promoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only