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Plasmid#227637PurposeNegative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertyfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYGS Q16C
Plasmid#217674PurposeFor expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experimentDepositorInsertCRYGS (CRYGS Human)
UseTags6XHis and SUMOExpressionBacterialMutationGlutamine 16 changed to CysteinePromoterT7Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXOOY
Plasmid#196449PurposeA dual vector for expression of membrane proteins in S. cerevisiae and X. laevisDepositorTypeEmpty backboneUseExpression in xenopus laevisTagsExpressionYeastMutationPromoteryeast CYC-GAL and T7 promoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Atg32-deltaPsR pXP721
Plasmid#200735PurposePlasmid for the expression of GFP-Atg32 lacking the PsR domain in S. cerevisiae.DepositorInsertAtg32
UseTagsGFPExpressionYeastMutationAmino acids 200-341 are deleted.PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg20-24 pST39
Plasmid#200439PurposeHis6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.DepositorInsertsAtg20
Atg24
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_B.1.640
Plasmid#180228PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the B.1.640 SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationB.1.640 (Amino Acid 319-869)PromoterT7Available sinceJan. 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_C.1.2
Plasmid#177367PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the C1.2 SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationC1.2 (Amino Acid 319-869)PromoterT7Available sinceJan. 20, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Mu
Plasmid#177366PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Mu SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationMu (Amino Acid 319-869)PromoterT7Available sinceJan. 20, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Kappa
Plasmid#175953PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Kappa SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationKappa (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Eta
Plasmid#175954PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Eta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationEta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Gamma
Plasmid#175047PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Gamma SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationGamma (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Theta
Plasmid#175951PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Theta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationTheta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Zeta
Plasmid#175952PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Zeta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationZeta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVW294
Plasmid#139491PurposeInsertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpGRE2 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpGRE2Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP569
Plasmid#139499PurposeRepair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl STL1 100 - 600
UseTagsExpressionYeastMutationWTPromoterpSTL1Available sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW295
Plasmid#139492PurposeInsertion of the pALD3 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpALD3 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpALD3Available sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP266
Plasmid#139488PurposeInsertion of the pCTT1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpCTT1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpCTT1Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP264
Plasmid#139487PurposeInsertion of the pSTL1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpSTL1Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW200
Plasmid#139489PurposeInsertion of the pGPD1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpGPD1 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpGPD1Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVW293
Plasmid#139490PurposeInsertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpHSP12 24xPP7sl
UseTagsExpressionYeastMutationWTPromoterpHSP12Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only