Showing: 10681 - 10700 of 16102 results
-
Plasmid#117416PurposeExpresses VMP1 tagged with 3xFLAG in mammalian cellsDepositorInsertVMP1
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP1
Plasmid#128306PurposeExpress hNABP1 (a.k.a hSSB2 or SOSSB2) in E. coli with a C-terminal His-tagDepositorInsertHuman nucleic acid-binding protein 1 (hNABP1) (NABP1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pDM304_YFP-IqgC
Plasmid#128697Purposeexpression of YFP-tagged IqgC in Dictyostelium discoideum cellsDepositorInsertiqgC
UseDictyostelium discoideum expression vectorTagsYFPExpressionMutationnonePromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP_∆R13–DD-talin1 (1-2299)
Plasmid#166125PurposeFor expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.DepositorInsert∆R13–DD-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationresidues 1-2299, deletion of dimerization domain …PromoterAvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA-Nr4a1
Plasmid#160941PurposeGeneration of retrovirus for the overexpression of Nr4a1DepositorInsertNr4a1 (Nr4a1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterMSCV-LTRAvailabilityAcademic Institutions and Nonprofits only -
SBP-ST*-GFP
Plasmid#163668PurposeExpresses SBP tagged partial length ST in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
UseTagsGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationThis chimera contains the first 113 amino acids o…PromoterAvailabilityAcademic Institutions and Nonprofits only -
FKBP-ST*-GFP
Plasmid#163669PurposeExpresses FKBP tagged partial length ST in mammalian cellsDepositorInsertpartial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
UseTagsFK506 binding protein (FKBP) and GFPExpressionMammalianMutationThis chimera contains the first 113 amino acids o…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-miniAAVR-FLAG
Plasmid#166717PurposeVector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tagDepositorInsertminiAAVR-FLAG
UseLentiviralTagsFLAGExpressionMammalianMutationOnly expressing PKD1-3 of ectodomainPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Ub-NLRP1 S1213
Plasmid#166827PurposepDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus (no stop)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsUbiquitinExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-hTBP
Plasmid#171073PurposeExpresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBPDepositorInsertTATA-box-binding protein (TBP Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
His-MBP_G4G6 in pRSF
Plasmid#188454PurposeExpress in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.DepositorInsertG4G6 (GSN Human)
UseTags6xHis-MBPExpressionBacterialMutationWTPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-JNKAR
Plasmid#181857PurposeGenetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-JNKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-EKAR
Plasmid#181858PurposeGenetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-EKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FluoSTEP-AKAR (C/Y)
Plasmid#181862PurposeFluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR (C-Y)
UseTagsCFP(1-10) and cpVenus(E127)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCDNA_MET_3Flag
Plasmid#182496PurposeContains cDNA of wt METDepositorInsertMET (MET Human)
UseTagsC-terminal 3xFlag tagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-TAPBPR-TM
Plasmid#178645PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TMDepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationswitched transmembrane domain with that of MHC-IPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pATZ-HA-GFP11
Plasmid#177720PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsGFP11 and HAExpressionBacterial and MammalianMutationE342K (ATZ mutant)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-HA-GFP11-KDEL
Plasmid#177722PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
UseTagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-DM-HA-GFP11-KDEL
Plasmid#177723PurposeDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFlucDM
UseTagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pHis-WzzE
Plasmid#196661PurposeExpression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
UseTagsTEV-cleavable His6ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 10681 - 10700 of 16102 results