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Plasmid#103088PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertB5XLC1(Cas9 coding gene from Streptococcus pyogenes serotype M49 (strain NZ131))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-C4ZA16
Plasmid#103090PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertC4ZA16(Cas9 coding gene from Eubacterium rectale (strain ATCC 33656 / VPI 0990))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSN29
Plasmid#100114Purposeexpresses a chimeric cMYC-repressor fusionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSN17
Plasmid#100104Purposeexpresses a chimeric OCT4-repressor fusionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
MT583
Plasmid#63114PurposeThe gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 expression in other genetic backgrounds.DepositorInsertACD6 (Accelerated Cell Death 6)
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_RBPMS2
Plasmid#65747Purposeexpresses GFP-tagged RBPMS2 in mammalian cellsDepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsGFPExpressionMammalianMutationS13N mutation in RBPMS2PromoterCMV, 2x TetAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
shCTL-mlpx-neo
Plasmid#65233Purposeencodes a non-targeting control shRNADepositorInsertnon-coding shRNA
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet28a_RBPMS_1_120
Plasmid#65737Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
UseTagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-120PromoterT7Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet28a_RBPMS_1_144
Plasmid#65739Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
UseTagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-144PromoterT7Available sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet23a_RBPMS_20_196
Plasmid#65734Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
UseTagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 20-196PromoterT7Available sinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet23a_RBPMS_1_129
Plasmid#65731Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
UseTagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-129PromoterT7Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet23a_RBPMS_1_111
Plasmid#65729Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
UseTagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 1-111PromoterT7Available sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet28a_RBPMS_20_196
Plasmid#65741Purposeplasmid for bacterial overexpression of RBPMSDepositorInsertRBPMS (RBPMS Human)
UseTagsHis TagExpressionBacterialMutationdeletion construct encoding amino acids 20-196PromoterT7Available sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR™221_RBPMS2
Plasmid#59389PurposepDONR vector with RBPMS2DepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsExpressionMutationPromoterT7Available sinceFeb. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Zyxin-6
Plasmid#57696PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertZyxin (ZYX Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mKO-Vinculin-23
Plasmid#57852PurposeLocalization: Focal Adhesions, Excitation: 548, Emission: 561DepositorInsertVinculin (VCL Human)
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Delta-ATG1-mMCL1
Plasmid#45823PurposeThis plasmid was made to show that the two MCL-1 bands DO NOT arise from a non-canonical translation initiation downstream of the start codon.DepositorInsertDelta ATG1-mMCL1
UseTagsExpressionMammalianMutationThe first amino acid (ATG) is deleted from the fu…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf200
Plasmid#12888PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf200
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3.loxP
Plasmid#63200PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K6.loxP
Plasmid#63203PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only