Addgene

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Ai32

Plasmid

#34880

Purpose

Cre-dependent expression of ChR2(H134R)-EYFP, for optogenetic excitation

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

Insert

Floxed ChR2(H134R)-EYFP

Use

Mouse Targeting

Tags

EYFP

Expression

Mutation

H134R in ChR2

Promoter

CAG

Available since

May 9, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

Plasmid

#164131

Purpose

Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_Ado1.0med

Plasmid

#153292

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

EFS

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

Plasmid

#118594

Purpose

Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

sgRNA/Puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-SOK

Plasmid

#20327

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-Oct4-T2A-Klf4 (Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-BiPOLES-mCerulean

Plasmid

#154950

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-Flag-SREBP1

Plasmid

#134283

Purpose

Expresses Flag-tagged SREBP1 in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP1 (Srebf1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

mouse SREBP1 isoform a precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His10FLAG

Plasmid

#157736

Purpose

empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

Depositor

Debra Hansen

Article

Olson et al Protein Expr Purif. 2021 May 7:105890. d

Type

Empty backbone

Use

Tags

His10 + 3xFLAG, enterokinase-cleavable

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV10-mFoxP1

Plasmid

#35170

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pTRIP-SFFV-mtagBFP-2A STING V155M

Plasmid

#102591

Purpose

Lentivector to express STING V155M and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

V155M

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Mb-sfGFP WT

Plasmid

#197569

Purpose

Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
Pyl-tRNA (4 copies)

Use

Tags

V5-His6

Expression

Mammalian

Mutation

Promoter

CMV and U6/H1

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Tead4

Plasmid

#128832

Purpose

dox-dependent expression of mouse Tead4

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Tead4 (Tead4 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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MAC-CTSB

Plasmid

#172413

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Cathepsin B (CTSB Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Promoter

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-Stop-NPM-ALK-MSNA-IRES-EGFP (MSNAIE)

Plasmid

#154470

Purpose

cDNA of NPM-ALK in the MSE backbone

Depositor

Cornelius Miething

Article

Shoumariyeh et al Oncogene. 2020 Feb;39(9):1904-1913. doi:

Insert

NPM-ALK

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-342STOP

Plasmid

#102588

Purpose

Lentivector to express STING delta 342 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

delta 342

Promoter

SFFV

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A23

Plasmid

#131988

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A23 (SLC22A23 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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LentiRCas9-CUG

Plasmid

#104183

Purpose

Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

Depositor

Eugene Yeo

Article

Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

Insert

U6-CUGsgRNA, EFS-PIN-dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTet-O-Nd1-puro

Plasmid

#52052

Purpose

Expresses Nd1 and puromycin resistent gene under control of TetON promoter

Depositor

Marius Wernig

Article

Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

Insert

Neurod1 (Neurod1 Mouse)

Use

Lentiviral

Tags

T2A-and puromycin resistant cassette

Expression

Mammalian

Mutation

Promoter

tet-on

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-mKOR

Plasmid

#179285

Purpose

Express mouse kappa opioid receptor (GFP tagged) in mammalian cells

Depositor

Christian Gruber

Article

Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055.

Insert

kappa opioid receptor (Oprk1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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