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Plasmid#205280PurposeSynaptic activity can be imaged with Synaptophysin GCaMP, while simultaneously, a red fluorescence structural marker can be employed to provide a visual reference for the cellular architecture.DepositorInsertGCaMP8s
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-mGreenLantern(2x)
Plasmid#197923PurposeHomo-dimer expression vector with plasma membrane localization signalDepositorInsertglycosylphosphatidylinositol-mGreenLantern-mGreenLantern
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TRAIL-R1
Plasmid#61382PurposeExpresses HA tagged TRAIL receptor 1DepositorInsertTRAIL receptor 1
UseTagsHA and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R1 leader peptide (aa1-23) replaced with TN…PromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti.pFOXA2.GFP
Plasmid#59404Purposeexpresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during developmentDepositorInsert400 bp FOXA2 promoter, eGFP (FOXA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterpFOXA2Available sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-MacroGreen
Plasmid#160665PurposeExpresses GFP fusion of a multisite mutant of the Af1521 proteinDepositorInsertAf1521 modified synthetic construct eGFP fusion
UseTagseGFP and hexahistidine_TEV recognitionExpressionBacterialMutationPromoterLacIAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCas
Plasmid#84232PurposeExpression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)DepositorInsertCas9
UseTags2A-OFP co-expression and ERT2-ERT2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
anti-FIX(a) Emi HC
Plasmid#113664PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FX(a) Emi HC and the Emi LC.DepositorInsertEmicizumab anti-FIX/FIXa heavy chain
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIMAY*
Plasmid#121441PurposeAllelic exchange plasmid with PheS* counter-selection for Staphylococcus aureusDepositorTypeEmpty backboneUseEscherichia coli/staphylococcus aureus shuttle ve…TagsExpressionMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt1(WT)
Plasmid#86637PurposeExpresses eGFP-tagged human Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP
Plasmid#205762PurposeSingle-color FUCCI4 senor for transitions of all four cell cylce phasesDepositorInsertrsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-mKate2-IRES-MCS (WT-WT)
Plasmid#105921PurposeAAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRsDepositorHas ServiceAAV2InsertsmKate2
IRES-Multiple cloning site
UseAAVTagsExpressionMutationcodon optimizedPromoterCBh and same CBh promoter as mKate2Available sinceFeb. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (AAV8)
Viral Prep#137163-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (#137163). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of ChR2-EYFP for optogenetic activation (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Cre-dependent)Available sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
GPR125-Tango
Plasmid#66313PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR125 (ADGRA3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Opie2-PuroR
Plasmid#131613PurposeExpresses PuroR and dsRed.DepositorInsertsPuroR
dsRed
UseTagsExpressionInsectMutationPromoterOpie2 and hr5Ie1Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-Aqp4-eGFP
Plasmid#176747PurposeExpresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytesDepositorInsertAqp4-eGFP
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgRosa26
Plasmid#159914PurposeMutagenesis of Rosa26 with SauCas9DepositorInsertRosa26 gRNA (Gt(ROSA)26Sor Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Active Wnt9A-V5
Plasmid#43820DepositorInsertWNT9A (WNT9A Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
C034
Plasmid#174340PurposeTemplate for making Rluc mRNA reporter with pA98.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-EA-BE4max-NG-BlastR
Plasmid#152992PurposeHigh purity C-to-T base editorDepositorInsertBE4 H47E+S48A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
EFSp-GFP-YAP
Plasmid#174168PurposeLentiviral vector to constitutively express YAP under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationPromoterEFSAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits