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Addgene
Showing: 10601 - 10620 of 32021 results

  3. pCas1_Cas2/3

    Plasmid
    #89240
    Purpose
    Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1
    Depositor
    Blake Wiedenheft
    Article
    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p
    Insert
    Cas1 and Cas2/3
    Use
    Tags
    Strep II tag on Cas1
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    89240-plasmid-map-sequence-id-176381

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  6. PDHA1-N-10

    Plasmid
    #58195
    Purpose
    Localization: Mitochondria, Excitation: N/A, Emission: N/A
    Depositor
    Michael Davidson
    Insert
    PDHA1 (PDHA1 Human)
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pENTR-R2L3-cGR-IRESpuro-pA

    Plasmid
    #139538
    Purpose
    Provides GR protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    GR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    139538-plasmid-map-sequence-id-274620

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  8. Vinculin-23

    Plasmid
    #58198
    Purpose
    Localization: Focal Adhesions, Excitation: N/A, Emission: N/A
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES

    Plasmid
    #138299
    Purpose
    Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    mCherry-PKCsub(T/A)-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138299-plasmid-map-sequence-id-307463

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  20. pcDNA3-HCMVgH-8his (Merlin)

    Plasmid
    #136447
    Purpose
    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    glycoprotein H (UL75 Human betaherpesvirus 5)
    Use
    Tags
    Short GGSG linker and 8his tag
    Expression
    Mammalian
    Mutation
    Extracellular domain (a.a. 1-716). Codon-optimize…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    136447-plasmid-map-sequence-id-273651

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Showing: 10601 - 10620 of 32021 results