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Plasmid#103237PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-140-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-140-5p target (MIR140 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-182-3p
Plasmid#103282PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-182-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-182-3p target (MIR182 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-150-3p
Plasmid#103256PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-150-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-150-3p target (MIR150 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA
Plasmid#68346PurposeUsed to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4DepositorInserts3xgRNA;PTENA, p53B,SMAD4A
FlPO
UseAAV; Flp/frtTagsExpressionMammalianMutationPromoterCAG and U6Available sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOXA10 (human) bluescript
Plasmid#8617DepositorInsertHOXA10 (HOXA10 Human)
UseTagsExpressionBacterialMutationimproved Ribosomal binding site (NcoI site) at AT…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-126-3p
Plasmid#103192PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-126-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-126-3p target (MIR126 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-22-3p
Plasmid#103363PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-22-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-22-3p target (MIR22 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K1S
Plasmid#62979PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseExpressionMutationFragment 1-446 of KLF4PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-125a-3p
Plasmid#103187PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-125a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-125a-3p target (MIR125A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-335-3p
Plasmid#103446PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-335-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-335-3p target (MIR331 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR2b-W290C
Plasmid#45700DepositorInsertFGFR2 IIIb (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationW290CPromoterAvailable sinceJune 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-495-5p
Plasmid#103569PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-495-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-495-5p target (MIR495 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-324-3p
Plasmid#103435PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-324-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-324-3p target (MIR324 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-324-5p
Plasmid#103436PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-324-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-324-5p target (MIR324 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-24-1-5p
Plasmid#103374PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-24-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-24-1-5p target (MIR24-1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-S37A-GATA6-3XAU1 puro
Plasmid#72608PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutationDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged serine37 to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ADAM7 (wt)
Plasmid#31139DepositorInsertADAM7 (ADAM7 Human)
UseTagsFLAGExpressionMammalianMutationwtPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb7-PIR-SH2
Plasmid#46443DepositorInsertGrb7 (GRB7 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 334-532PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-PRAS40
Plasmid#53599Purposelentiviral expression of constitutively active PRAS40 and EGFPDepositorInsertPRAS40 (AKT1S1 Human)
UseLentiviralTagsIRES-EGFP and mycExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat2-SH2
Plasmid#46512DepositorInsertStat2 (STAT2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 566-681PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only