-
Plasmid#192349PurposeExpresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive formDepositorInsertSARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
UseTags6X-His-SumoExpressionBacterialMutationCysteine 145 of the active site is substituted wi…PromoterT7 lacAvailable sinceJune 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT7C-HSP90AB1
Plasmid#177659PurposeRecombinant protein synthesis of human HSP90AB1DepositorInsertHSP90AB1 (HSP90AB1 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-ActA-pEGFP-C1
Plasmid#177405PurposeTo express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTag-BFP-C-h-Rab7L1-c-Myc
Plasmid#79803PurposeExpress TagBFP labeled human Rab7L1.DepositorInsertRab7L1 (RAB29 Human)
UseTagsTagBFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP VAMP8
Plasmid#42311DepositorInsertVAMP 8 (VAMP8 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSR660
Plasmid#65216PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-8 cap
UseTagsExpressionInsectMutationPromoterP10 and polyhedrinAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-MjDim1
Plasmid#189936PurposeExpression of Mjdim1, a methyltransferase used in m6A-SAC-seq.DepositorInsertrsmA (MJ_RS00455 Methanocaldococcus jannaschii)
UseTags6x His tag, FLAG tag, SUMO tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDL025_HA-rb_IgG_HC_15F11
Plasmid#193622PurposeHA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.DepositorInsertAnti_HA_15F11 rabbit heavy chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL026_HA-rb_IgG_LC_15F11
Plasmid#193623PurposeHA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.DepositorInsertAnti-HA_15F11 rabbit light chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-p38alpha
Plasmid#86832Purposemammalian expression of GFP-p38alphaDepositorInsertp38alpha (MAPK14 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-3xFLAG
Plasmid#69837PurposeThis plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
CaV2.1 (pcDNA6)
Plasmid#26578DepositorInsertCacna1a (Cacna1a Mouse)
UseTagsHis6 and V5ExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC scAB-GFP
Plasmid#104998PurposeLentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labelingDepositorInsertsingle chain Antibody against GCN4
UseLentiviralTagsGB1, HA tag, and sfGFPExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI neo-hEST2
Plasmid#1781DepositorInserthTERT (TERT Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
UseTagsExpressionMammalianMutationA191GPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_empty
Plasmid#99297PurposeLuciferase validation vector; empty vector without insert to determine baselineDepositorTypeEmpty backboneUseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMax_GFP_C289T
Plasmid#177826PurposeSite-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).DepositorInsertGFP_C289T
UseTagsExpressionMammalianMutationC289TPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-CBh-eGFP
Plasmid#206885Purposecloning backbone for sgRNA using OptScf2DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterU6Available sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-ARPC3
Plasmid#31580DepositorInsertActin related protein 2/3 complex, subunit 3 (ARPC3 Human)
UseTagsHis and StrepII-tagExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLX-B2 (GB3374)
Plasmid#160636PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only