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pGST-DHFR Mm

Plasmid

#83073

Purpose

Expresses the full length DHFR Mm in E.coli

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

2x DHFR (Dhfr Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-BATF

Plasmid

#178451

Purpose

doxycycline-inducible expression of Human BATF in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

BATF (BATF Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pALS2-sfGFP 150TAG

Plasmid

#197574

Purpose

Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
M. alvus Pyl-tRNA (6)

Use

Tags

His6

Expression

Bacterial

Mutation

N150TAG

Promoter

araC and lpp

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti PGK mKate2-OGT K842M (Puro)

Plasmid

#154291

Purpose

Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x FLAG and mKate2

Expression

Mammalian

Mutation

K842M mutation (Catalytic Inactive)

Promoter

PGK

Available since

Dec. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC6A6

Plasmid

#132196

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A6 (SLC6A6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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mAC-POLR2A donor (Hygro)

Plasmid

#124496

Purpose

A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover

Depositor

Masato Kanemaki

Article

Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

Insert

POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)

Use

CRISPR

Tags

mAID-mClover

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET-45b-POLR2A-CTD-mCherry

Plasmid

#185011

Purpose

Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

POLR2A (Polr2a Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKO-2TO

Plasmid

#86697

Purpose

Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

H1 2TO

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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miniCGBE1-SpRY (pBM1571)

Plasmid

#170105

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

Use

CRISPR

Tags

P2A-EGFP

Expression

Mammalian

Mutation

R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

Promoter

CMV

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-EFIa-Id1-PGK-puro

Plasmid

#107735

Purpose

Expresses Id1 in mammalian cells

Depositor

Alexandre Colas

Article

Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

Insert

Id1 (T Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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TH1204-pOCC120-FUS_opt(NheI)

Plasmid

#221892

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Use

Tags

MBP-PreScission and mGFP-PreScission-6xHis

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223249

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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TH1258-013_Jie_TAF15_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP

Plasmid

#221893

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

TAF15 (TAF15 Human)

Use

Tags

6xHis-MBP-PreScission and TEV-mGFP

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-eLACCO2.1-NGR

Plasmid

#208023

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC47A1

Plasmid

#132227

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC47A1 (SLC47A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

Plasmid

#86613

Purpose

Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR; Co-selection via hdr using ouabain

Tags

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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hSyn-BiPOLES-mCerulean

Plasmid

#154944

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLV V5-lamin A

Plasmid

#175107

Purpose

Lentiviral expression of V5-tagged mouse Lmna

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Lmna (Lmna Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#216249

Purpose

Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

Cas9 and sgRANGAP.C1 (RANGAP1 Human)

Use

CRISPR; Endogenous tagging

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC41A1

Plasmid

#132237

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC41A1 (SLC41A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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