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Plasmid#83407Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationC29A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1-V28A
Plasmid#83406Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationV28A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C(1-686)AVITEV
Plasmid#74054Purposeretroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
UseRetroviralTagsAVI-TEVExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HISX6-TEV-RSP4
Plasmid#176693PurposeExpression of Chlamydomonas reinhardtii RSP4 recombinant protein in insect cellsDepositorInsertRSP4
UseTagsHISX6ExpressionInsectMutationNonePromoterpolyhedrinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_GLP1R-V2R-NTEV-TCS-GV-2xHA
Plasmid#194380PurposeSplit TEV assaysDepositorInsertGLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-super TEV protease
Plasmid#171782PurposeExpresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.DepositorInsertMBP-sTEV
UseTagsArg6 tag and Internal His6 tagExpressionBacterialMutationNo internal TEV cleavage site between the MBP and…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL1 CTF-FLAG
Plasmid#217706PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL1 (ADGRL1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-838PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-M13-TEV-C-P2A-TdTomato
Plasmid#92391Purposeproduces AAV expressing Cal-light M13-TevC component and TdTomatoDepositorInsertM13-TEV-C-P2A-TdTomato
UseAAV and Synthetic BiologyTagsTdTomatoExpressionMammalianMutationPromoterhuman synapsinAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-16b-10His-TEV-MCES_VACCA_D1_D12
Plasmid#203671PurposeThe D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavageDepositorInsertMCES_VACCA_D1_D12
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIR-His-Halo-Tev-PTEN
Plasmid#58241Purposeexpresses halo tagged PTEN proteinDepositorInsertphosphatase and tensin homolog (PTEN Human)
UseTagsHis-Halo-TevExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CARD8-TEV-GFP-FLAG
Plasmid#169991PurposeMammalian purification construct for CARD8DepositorInsertCARD8 (CARD8 Human)
UseTagsTEV-GFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-MBP-TEV-NAP1 (WT)
Plasmid#208871PurposeFor expression in E. coli of NAP1DepositorInsertNAP1 (NAP1L1 Human)
UseTagsMBP-TEVExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRM055 His-TEV-Cas9 6xNLS 0C
Plasmid#196246PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
UseTags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDY0216 PASTEv1 PE2-P2A-Bxb1
Plasmid#179104PurposePASTEv1 PE2-P2A-Bxb1DepositorInsertpCMV-PE2-P2A-Bxb1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS
Plasmid#158741PurposeExpresses influenza A virus trimeric hemagglutinin A/H1/MI/15 fused to a sfGFPDepositorInsertHemagglutinin A/MI/45/15 H1N1
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationAA61-469PromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16
Plasmid#158742PurposeExpresses influenza A virus tetrameric neuraminidase fused to a sfGFPDepositorInsertNeuraminidase A/NL/354/16 H3N2
UseTagsTwinStrep_sfGFP_tetrabrachionExpressionMammalianMutationAA61-469PromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-ORF9B
Plasmid#154425PurposeGateway-compatible Entry vectorDepositorInsertORF9B
UseGateway-compatible entry vector, with insert of o…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCD5-H3CH13-CO-GCN4-TEV-sfGFP-TS
Plasmid#158740PurposeExpresses influenza A virus trimeric hemagglutinin A/H3/CH/13 fused to a sfGFPDepositorInsertHemagglutinin A/MI/45/15 H1N1
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationAA61-469PromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-ORF3A
Plasmid#154416PurposeGateway-compatible Entry vectorDepositorInsertORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of o…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceSept. 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits