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Plasmid#89918PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSB and FSC (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only
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BB2_L_CD_syn_BbsI
Plasmid#89919PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSC and FSD (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_DE_syn_BbsI
Plasmid#89920PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSD and FSE (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_FG_syn_BbsI
Plasmid#89922PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSF and FSG (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_GH_syn_BbsI
Plasmid#89923PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast
Plasmid#26655Purpose3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
p5xATF6-GL3
Plasmid#11976PurposeReporter gene with five ATF6 sites that is highly sensitive to ER stressDepositorInsert5xATF6 binding site
UseTagsLuc and c-fos promoterExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
5HT6-GCaMP5G
Plasmid#48340PurposeFluorescent reporter for Ca2+ inside primary ciliaDepositorInsertGCaMP5G
UseTags5HT6ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMVlux-cp157Venus
Plasmid#197584PurposeBRET lux luciferase with cp157Venus for mammalian expressionDepositorInserthuman codon-optimized Lux-cp157Venus
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
luxB:cp157Venus+luxA/pRSETB Duet
Plasmid#197583PurposeBRET lux luciferase with cp157Venus for bacterial expressionDepositorInsertLuxA LuxBcp157Venus
UseE.coli expressionTagsHis X 6ExpressionBacterialMutationPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-TIS11B
Plasmid#121157PurposeMammalian expression of GFP tagged TIS11BDepositorInsertZFP36L1 (TIS11B) (ZFP36L1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-shNT
Plasmid#109012Purposeshort-hairpin knockdown of targeted geneDepositorInsertNon-targeting shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
GW1-ARSeNL
Plasmid#207873PurposeMammalian expression of the BRET ATP sensorDepositorInsertARSeNL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GCEPIA1-SNAPER
Plasmid#172659PurposeDetect the real-time ER calcium store and local calcium microdomains in live cells.DepositorInsertcalmodulin
UseTagsExpressionMammalianMutationPlease see depositor commentsPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER_K0
Plasmid#133863PurposeExpression of ER-targeted Ribosome non-stalling reporterDepositorInsertER_K0
UseTagsEGFP and RFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-TIS11B
Plasmid#121140PurposeMammalian expression of mCherry tagged TIS11BDepositorInsertZFP36L1 (TIS11B) (ZFP36L1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cyto_K0
Plasmid#133864PurposeExpression of cytoplasmic Ribosome non-stalling reporterDepositorInsertCyto_K0
UseTagsEGFP and RFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
luxA+luxB/pRSETB Duet
Plasmid#197581PurposeExpressing luxA and luxB under dual T7 promoterDepositorInsertLuxA LuxB
UseTagsT7 tag and 6xHisExpressionBacterialMutationPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPM47 (UPR-RFP CEN/ARS URA3)
Plasmid#20132DepositorInsertUPR-RFP
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2009AvailabilityAcademic Institutions and Nonprofits only