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Showing: 1041 - 1060 of 2423 results

  1. pUltra-dox

    Plasmid
    #58749
    Purpose
    doxycycline inducible multicistronic lentiviral gene expression system.
    Depositor
    Kitai Kim, Yildirim Dogan
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE2 upstream of minimal CMV promoter
    Available since
    Aug. 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    58749-plasmid-map-sequence-id-92491

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  2. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

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  3. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

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  4. pcDNA-dCas9-p300 Core (Y1467F)

    Plasmid
    #61362
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; Y1467F mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61362-plasmid-map-sequence-id-99796

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  5. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid
    #61361
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61361-plasmid-map-sequence-id-99794

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  6. OpenCRISPR-1 sgRNA-16nt stem

    Plasmid
    #221566
    Purpose
    Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
    Depositor
    Ali Madani
    Insert
    OpenCRISPR-1 sgRNA 16nt stem loop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221566-plasmid-map-sequence-id-437904

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  7. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid
    #221567
    Purpose
    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
    Depositor
    Ali Madani
    Insert
    OpenCRISPR-1 sgRNA 12nt stem loop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221567-plasmid-map-sequence-id-437907

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  9. pNHA_SBV_Gc

    Plasmid
    #208933
    Purpose
    Expresses N-terminally HA-tagged Schmallenberg orthobunyavirus Gc protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    Gc
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 1682 bp from 5' end, deleted 378 bp …
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208933-plasmid-map-sequence-id-419021

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  12. enIscB

    Plasmid
    #205410
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E85R+H369R+S387R+S457R
    Promoter
    CAG, hU6, CMV
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    205410-plasmid-map-sequence-id-421947

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  13. enIscB-T5E

    Plasmid
    #205411
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E85R+H369R+S387R+S457R
    Promoter
    CAG, hU6, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205411-plasmid-map-sequence-id-416557

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  16. pCDH-DCX-TurboID-V5-puro

    Plasmid
    #190742
    Purpose
    A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)
    Depositor
    Jeremy Baskin
    Article
    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021
    Insert
    DCX-TurboID-V5 (DCX DCX is human doublecortin; TurboID is engineered BirA from E.coli, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    190742-plasmid-map-sequence-id-379321

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  17. PB-iRFP-STOP-ReNL

    Plasmid
    #113965
    Purpose
    Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)
    Depositor
    Jordan Green
    Article
    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:
    Inserts
    iRFP670
    ReNL
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV - SV40-PolyA - SV40-PolyA and EF1A
    Available since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    113965-plasmid-map-sequence-id-223037

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  18. AdMKP-3 S159AS197A

    Plasmid
    #131523
    Purpose
    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells
    Depositor
    Haiyan Xu
    Article
    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2
    Insert
    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    Use
    Adenoviral
    Tags
    No
    Expression
    Mutation
    changed Serine 159 to Alanine and Serine 197 to A…
    Promoter
    CMV
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    131523-plasmid-map-sequence-id-256915

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Showing: 1041 - 1060 of 2423 results