Addgene

We narrowed to 1,350 results for: MiCy

Showing: 1041 - 1060 of 1350 results

Results per page:

Sort results by:

pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

Plasmid

#79031

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(African green monkey pygerythrus)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFBD-Akt1(D323A/D325A)

Plasmid

#86592

Purpose

Expresses human full-length Akt1(D323A/D325A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

10xHis

Expression

Insect

Mutation

D323A/D325A transforming mutations

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pfast bac-flag Fbxl3

Plasmid

#51894

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl3 (Fbxl21 Mouse)

Use

Tags

FLAG

Expression

Mutation

Promoter

polyhedrin promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pFast-His6-hRad17

Plasmid

#222465

Purpose

Expresses hRad17 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hRad17 (RAD17 Human)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFast-Flag-hRad9

Plasmid

#222468

Purpose

Expresses hRad9 in insect cells

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hRad9 (RAD9A Human)

Use

Tags

Flag epitope

Expression

Insect

Mutation

Promoter

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACE SOX (ORF37) D221N E244Q

Plasmid

#192053

Purpose

Expresses a 2X catalytic mutant of (ORF37) D221N E244Q

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (D221N E244Q)

Use

Unspecified

Tags

Expression

Mutation

2X catalytic mutant D221N E244Q

Promoter

CMV

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCOLA-Gent-EM7-Erv1p-DsbC

Plasmid

#202485

Purpose

Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

EM7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCOLA-Gent-EM7-Erv1p-PDI

Plasmid

#202486

Purpose

Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Human, Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

EM7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKC31

Plasmid

#226777

Purpose

Expresses Drosophila UNC-104(R562H) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (R562H) (unc-104 Fly)

Use

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Arginine 562 to Histidine (bris mutation)

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG068

Plasmid

#214259

Purpose

pJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaI

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M

Use

Tags

Expression

Bacterial

Mutation

18 nucleotide 5' of the start codon (Shine-D…

Promoter

araBAD promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL1318

Plasmid

#217960

Purpose

Expression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H4 (H4C1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

L63A, F66A, I71A

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL1317

Plasmid

#217959

Purpose

Expression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H4 (H4C1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

GGG insertion between V65 and F66

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC5GGv1_ShBle

Plasmid

#188603

Purpose

pSC5GGv1 with Sh ble insert for yeast selection

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT 3xFLAG-BCAS2

Plasmid

#192294

Purpose

Gateway entry vector for an inducible 3xFLAG-BCAS2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BCAS2 (BCAS2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT 3xFLAG-DYNLL2

Plasmid

#192296

Purpose

Gateway entry vector for an inducible 3xFLAG-DYNLL2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

DYNLL2 (DYNLL2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
M2C5

Plasmid

#188606

Purpose

Minimal Conjugative Plasmid 2

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
M1C1

Plasmid

#188609

Purpose

Minimal Conjugative Plasmid 1

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pACE-K2P4.1-StrepTagII

Plasmid

#191474

Purpose

Expresses K2P4.1 with C-terminal StrepTagII

Depositor

Arthur Laganowsky

Article

Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

Insert

K2P4.1 (KCNK4 Human)

Use

Tags

Expression

Insect

Mutation

N104Q N108Q

Promoter

polH

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR2_+10D+7U

Plasmid

#149393

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

7bp inserted upstream, and 10bp inserted downstre…

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR2_+10D+10U

Plasmid

#149394

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

10bp inserted upstream and downstream of the IHFp…

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge