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pLITMUS-rpoN-cI5G6G,P-J23106-geneIII

Plasmid

#80861

Purpose

Phagemid (encodes evolved cI5G6G,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6G,P

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pTNT-pT7-tetR-sfGFP

Plasmid

#140868

Purpose

T7 promoter driven synthesis of C-terminal fusion of sfGFP to TetR repressor (TetR-sfGFP)

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-sfGFP expressed with T7 promoter

Use

Synthetic Biology

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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SPELL-Lyn Component 1

Plasmid

#170484

Purpose

SPELL Lyn kinase (Constitutively active) N-lobe fused to iFKBP. Split site is 279.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

Lyn(N-lobe), iFKBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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SPELL-Lyn Component 2

Plasmid

#170485

Purpose

SPELL Lyn kinase (Constitutively active) C-lobe fused to FRB. Split site is 279.

Depositor

Klaus Hahn

Article

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

FRB, Lyn(C-lobe)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pIM23

Plasmid

#74643

Purpose

Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Use

Tags

Expression

Yeast

Mutation

Promoter

MET25

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5G6T,P-J23106-geneIII

Plasmid

#80862

Purpose

Phagemid (encodes evolved cI5G6T,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6T,P

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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RECQL5 (RECQL5A-c104)

Plasmid

#98234

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

RECQL5A (RECQL5 Human)

Use

Tags

His-6-TEV

Expression

Bacterial

Mutation

pfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…

Promoter

T7

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pUG-UBC2

Plasmid

#104667

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC2 (UBC2 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII

Plasmid

#80863

Purpose

Phagemid (encodes evolved cI4A5T6T,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5T6T,P

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5G6G-J23106-geneIII

Plasmid

#80906

Purpose

Phagemid (encodes cI5G6G and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6G

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5G6T-J23106-geneIII

Plasmid

#80907

Purpose

Phagemid (encodes cI5G6T and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6T

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI4A5T6T-J23106-geneIII

Plasmid

#80908

Purpose

Phagemid (encodes cI4A5T6T and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5T6T

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-rsAKARev

Plasmid

#166231

Purpose

Expresses reversibly switchable A-kinase activity reporter with EV-linker (rsAKARev) in mammalian cells

Depositor

Peter Dedecker

Article

Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

Insert

Reversibly switchable A-kinase activity reporter with EV-linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pH6Z-101

Plasmid

#85226

Purpose

Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

Depositor

Philip Pellett

Article

Lindquester et al Arch Virol. 1996;141(2):367-79.

Insert

fragment of the human herpesvirus 6B strain Z29 genome

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFDP1

Plasmid

#67084

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFDP1 (TFDP1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
mPlum-Alpha-Actinin-19

Plasmid

#55949

Purpose

Localization: alpha Actinin, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

mPlum

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only
PGK mScarlet-Synaptophysin

Plasmid

#206145

Purpose

PGK mScarlet-Synaptophysin

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

synaptophysin (Syp Rat)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

PGK

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PGK EGFP-RAB3A-WT

Plasmid

#206146

Purpose

PGK EGFP-RAB3A-WT

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

RAB3A (RAB3A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-Dbp

Plasmid

#186822

Purpose

Fluorescent reporter for Dbp expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

dbp promoter (Dbp Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

dbp promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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mouse KANK4 Ankyrin Repeats pET151

Plasmid

#164057

Purpose

Expresses mouse KANK4 Ank domain in bacterial cells

Depositor

Ben Goult

Insert

Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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