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pLVpuro-CMV-N-3xFLAG

Plasmid

#123223

Purpose

N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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IL6R PLAK1

Plasmid

#161518

Purpose

Expresses IL6 receptor (full length) in mammalian cells.

Depositor

Yoav Shaul

Article

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

Interleukin 6 Receptor (IL6R Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLL5.0-MRLC-DD-GFP

Plasmid

#187260

Purpose

Lentiviral expression of myosin regulatory light chain-DD mutant

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

T18D, S19D

Promoter

5' LTR

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0-MRLC-AA-GFP

Plasmid

#187259

Purpose

Lentiviral expression of myosin regulatory light chain-AA mutant

Depositor

Alpha Yap

Article

Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

T18A,S19A

Promoter

5' LTR

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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Snail_pGL2

Plasmid

#31694

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Snail (SNAI1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pRSET-mEosEM

Plasmid

#132708

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mEosEM

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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peYFP-C1-mTOR

Plasmid

#73384

Purpose

Expresses YFP tagged mTOR

Depositor

Taekjip Ha, Jie Chen

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

mTOR (MTOR Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur

Plasmid

#60523

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSFFV-SpyTag-sfCherry2(11)-TagBFP

Plasmid

#117485

Purpose

Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyTag002-GG-sfCherry2(11)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pIRE4-Bpa

Plasmid

#155342

Purpose

Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

Depositor

Irene Coin

Article

Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

Insert

humanized EBpaRS/BstYam

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK/U6

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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