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Plasmid#208027PurposeBiosensor for intracellular L-lactateDepositorInsertR-iLACCO1.2
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-R-iLACCO1.1
Plasmid#208026PurposeBiosensor for intracellular L-lactateDepositorInsertR-iLACCO1.1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBK- Ma PylRS WT
Plasmid#197573PurposeExpresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS WT
UseTagsExpressionBacterialMutationPromoterGlnS (constitutive)Available sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_R248W
Plasmid#183114PurposeExpresses flag-tagged p53 with R248W mutation in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchanged arginine 248 to tryptophanPromoterEF1aAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-GFP
Plasmid#181906PurposeFluorescently tagged dead Cas9DepositorInsertdCas9
UseCRISPRTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
UseTagsExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Myc2- Hs TTP WT
Plasmid#107008PurposeExpresses epitope-tagged wild-type human TTPDepositorInserttristetraprolin (ZFP36 Human)
UseTagsDouble Myc peptide epitope tagExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP core
Plasmid#179543Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoterDepositorInsertS. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRM18 LTN (Luciferase tdTomato Neo)
Plasmid#174721PurposeExpresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.DepositorInsertsFirefly Luciferase
tdTomato
aph
UseLentiviral and LuciferaseTagsE2A linker and P2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-iLACCO1.1
Plasmid#208100PurposeBiosensor for intracellular L-lactateDepositorInsertR-iLACCO1.2
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gfaABC1D-R-iLACCO1.1
Plasmid#208103PurposeBiosensor for intracellular L-lactateDepositorInsertdeLACCO1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216246PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integrationDepositorInsertCas9 and sgAAVS1.5
UseCRISPR; Human safe harbor locus (a avs1) site-spe…TagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-BI-AR
Plasmid#171234PurposeMammalian expression of 2xHA-tagged human androgen receptor (hAR)DepositorInsert2xHA-tagged human androgen receptor (AR Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro
Plasmid#216244PurposeExpress AtAFB2.F74A mutant with mCherry tag for AID2DepositorInsertAtAFB2.F74A-mCherry
UseCRISPR, TALEN, and Unspecified ; Human safe harbo…TagsmCherryExpressionMammalianMutationF74A mutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-R-diLACCO1
Plasmid#208028PurposeControl biosensor for intracellular L-lactateDepositorInsertR-diLACCO1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(MC1-42)
Plasmid#127151PurposeExpresses N-terminal cysteine Abeta(1-42)DepositorInsertAbeta(MC1-42) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/avitag-apex2-v5-fkbp8
Plasmid#154460Purposeexpresses avitag-apex2-v5-fkbp8/biochemical assayDepositorInsertavitag-apex2-v5-fkbp8 (FKBP8 Human)
UseTagsExpressionMammalianMutationPromoterCMV-F, T7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcrDNA3.1-PRKACA
Plasmid#100890PurposeMammalian expression of PRKACADepositorInsertProtein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
UseTagsExpressionMammalianMutationIsoform 1 wild typePromoterCMVAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only