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Plasmid#216276PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1aDepositorInsertNES-jRGECO1a
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman synapsin 1 (hSyn)Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP-C-IFT74
Plasmid#218730PurposeExpresses N-terminally EGFP-tagged IFT74 in mammalian cellsDepositorInsertIFT74 (IFT74 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT43
Plasmid#218735PurposeExpresses N-terminally EGFP-tagged IFT43 in mammalian cellsDepositorInsertIFT43 (IFT43 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
Plasmid#217509PurposeKD Recombinase dependent conditional 1X ALFA cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
UseTagsALFA tagExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMV10 lenti pEF-rTetR(SE-G72P)-3XFLAG-WWP2(WW)-T2A-mCherry-BSD-WPRE
Plasmid#188756PurposeLentiviral backbone containing WWP2 WW domain as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertWWP2 WW (WWP2 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT151 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF10 KRAB-T2A-mCherry-BSD-WPRE
Plasmid#188757PurposeLentiviral backbone containing ZNF10 KRAB as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertZNF10 KRAB (ZNF10 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT228 lenti pEF-rTetR(SE-G72P)-3XFLAG-KIBRA WW-T2A-mCherry-BSD-WPRE
Plasmid#188762PurposeLentiviral backbone containing KIBRA WW as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertKIBRA WW (WWC1 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188747PurposeDonor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorArticleInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterUbCAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMV2 AAV-RapaInducible-NFZ-HGF
Plasmid#188749PurposeRapamycin inducible AAV vector expressing HGFDepositorArticleInsertHGF (HGF Human)
UseAAVTagsExpressionMammalianMutationPromoterRapamycin inducibleAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMV8 lenti pEF-rTetR(SE-G72P)-3XFLAG-PYGO1(PHD)-T2A-mCherry-BSD-WPRE
Plasmid#188754PurposeLentiviral backbone containing PYGO1 PHD domain as a fusion with rTetR(SE-G72P)-3XFLAGDepositorArticleInsertPYGO1 PHD (PYGO1 Human)
UseLentiviralTagsrTetR(SE-G72P)-3xFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188743PurposeDonor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorArticleInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterRSVAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188744PurposeDonor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorArticleInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGKAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188745PurposeDonor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorArticleInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoternonTATAchr21Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188746PurposeDonor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorArticleInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoternonTATAchrXAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT241 Lenti pEF-dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast
Plasmid#187326PurposedCas9 effector fusion for manipulating transcriptionDepositorArticleInsertdCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT244 Lenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast
Plasmid#195537PurposedCas9 effector fusion for manipulating transcriptionDepositorArticleInsertLenti pEF-dCas9-3XFLAG-RYBP-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT342 Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
Plasmid#195541PurposedCas9 effector fusion for manipulating transcriptionDepositorArticleInsertLenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianMutationPromoterpEF1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-SF14P
Plasmid#209448Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from SF14P promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-kasOP*
Plasmid#209447Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from kasOP* promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Luciferase-CD4-bla
Plasmid#189981PurposeDonor Vector to integrate firefly Luciferase C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertLuciferase
UseCRISPRTags3xFlagExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only