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Plasmid#60615PurposeEncodes YFP and NLS tagged beta-actin with the G13R depolymerization mutationDepositorInsertBeta-actin (ACTB Human)
UseTagsEYFP and NLSExpressionMammalianMutationG13RPromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP hsp70 K71E
Plasmid#15216DepositorInserthsp70 K71E (HSPA1A Human)
UseTagsEGFPExpressionMammalianMutationK71E, ATPase-deficient mutant.PromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-DVL1-WT
Plasmid#194586PurposeMammalian Expression of mEGFP-DVL1 WT Fusion ProteinDepositorInsertDVL1 (DVL1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TSHR-Tango
Plasmid#66518PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertTSHR (TSHR Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-GFP
Plasmid#87662Purposeretroviral vector encoding cre dependent expression of GFPDepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterCAGAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3
Plasmid#112007PurposeFor co-expression of GCaMP6s with mRuby3DepositorHas ServiceAAV9InsertGCaMP6s-P2A-mRuby3
UseAAVTagsExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LiOn*-CAG∞RFP
Plasmid#154019PurposeVector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
UseTagsExpressionMammalianMutationAll TTAA in vector backbone were replaced by sile…PromoterCAGAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCT2
Plasmid#45799DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP Snail WT
Plasmid#16225DepositorInsertSnail (SNAI1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-A KD/MD
Plasmid#104449PurposeExpression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein A (VAPA Human)
UseTagsEGFPExpressionMammalianMutationchanged K94 to D and M96 to DPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSAG1::CAS9-U6::sgUPRT
Plasmid#54467PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.DepositorInsertSAG1 5' UTR
UseCRISPR; Toxoplasma expressionTagsExpressionMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABEbleo-Flag-BRAFV600E
Plasmid#53156PurposeExpresses Flag tagged human oncogenic BRAFV600E from bleomycin resistance retroviral vectorDepositorInsertBRAF (BRAF Human)
UseRetroviralTagsFLAGExpressionMammalianMutationV600EPromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-fDIO-GFP-IRES-tTA
Plasmid#118026PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-fDIO-GFP-IRES-tTA
UseAAVTagsExpressionMutationSee depositor comments belowPromoterTREAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)
Plasmid#138555PurposeExpresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSDDepositorInserteSpCas9(1.1)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationK848A, K1003A, R1060APromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHrD-IRES-Fluc
Plasmid#194250PurposeHuman RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterhuman rDNA promoter and beginning of 5' ETS …Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJM.336
Plasmid#108528PurposeLacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.DepositorInsertLacIAM + PTac-YFP
UseTagsExpressionBacterialMutationLacI Q18M A47V F161YPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Luc-T20
Plasmid#177941PurposeTransient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInsertLuc2 (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS
Plasmid#111849PurposeExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
3xflag-APEX2-LRRK2
Plasmid#164622PurposeExpresses LRRK2 tagged with APEX2 and 3xFLAGDepositorInsertLRRK2 (LRRK2 Human)
UseTags3xFLAG and APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTR-346 pUC19-tNGFR-P2A-IL2RA N
Plasmid#186065PurposeKnockin of truncated NGFR to target geneDepositorInsertIL2RA (IL2RA Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only