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Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDHt-KS
Plasmid#63616PurposeThe multiple cloning site of pGreenII0000 cloned into binary vector pDHtDepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSP571
Plasmid#139498PurposeCRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNADepositorInsertpGPD Cas9 / sgRNA (STL162)
UseTagsExpressionYeastMutationWTPromoterpGPDAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 YWHAB
Plasmid#67063PurposeStandard for cell-free expression benchmarking.DepositorInsertYWHAB (YWHAB Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A_dCas9-1x5
Plasmid#63599PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA2
Plasmid#64984PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A_dCas9-1x3
Plasmid#63597PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAP72_MS2
Plasmid#220623PurposeCassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.DepositorTypeEmpty backboneUseShuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0520 (pDEST, TRP1)
Plasmid#210481PurposepDEST vector with TRP1 marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseDestination vector for gateway cloningTagsExpressionMutationPromoterADH1Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0604 (pDEST, HygR)
Plasmid#210483PurposepDEST vector with HygR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0603 (pDEST, NatR)
Plasmid#210482PurposepDEST vector with NatR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPSA5Cas9syn
Plasmid#206965PurposeS. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGTDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg24 1B
Plasmid#197046PurposeHis6 tagged Atg24 from S. cerevisiae for expression in E. coli.DepositorInsertAtg24
UseTags6His-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg32 [200-341] 1B
Plasmid#190431PurposeRecombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.DepositorInsertAtg32
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg11 [699-800] pHis2
Plasmid#190264PurposeCodon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.DepositorInsertATG11
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce-Prepro SP
Plasmid#153491PurposeS. cerevisiae Prepro Signal Peptide Donor PlasmidDepositorInsertPrepro SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCuHA-SpAtg101(414)
Plasmid#122081PurposeExpressing S. pombe HA-Atg101DepositorInsertSpATG101
UseTags3HAExpressionYeastMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-RAMA
Plasmid#112226PurposeExpresses RAMA domain from murine MPNDDepositorInsertMPND (Mpnd Mouse)
UseTagsFlag and HIs S-tagExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5woH
Plasmid#65019PurposeExpresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureusDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA2woH
Plasmid#65018PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromotertetracycline promoter/operatorAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only