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pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

Plasmid

#141395

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

eGFP

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDIRECT_25F

Plasmid

#91143

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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MTDH-His-bio

Plasmid

#52331

Purpose

Expresses full-length Metastasis adhesion protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

MTDH (MTDH Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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mEmerald-Keratin-17

Plasmid

#54134

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Keratin (KRT18 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-Rab35 S22N inactive

Plasmid

#47426

Depositor

Peter McPherson

Article

Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

Insert

Rab35 S22N (RAB35 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

S22N inactive

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pEVS104

Plasmid

#207393

Purpose

Conjugal helper plasmid

Depositor

Jason Peters, Eric Stabb, Edward Ruby

Article

Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_FLUAV_HA

Plasmid

#207273

Purpose

Mammalian expression of Influenza A virus HA protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

FLUAV_HA (HA Influenza A virus)

Use

Tags

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-mCherry (AAV8)

Viral Prep

#137134-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-mCherry (#137134). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-mCherry plasmid DNA. EF1a-driven, Flp-dependent expression of mCherry (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Promoter

EF1a

Tags

mCherry (Flp-dependent)

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only
pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84241

Purpose

Expresses ABA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Mutation

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-CTCF

Plasmid

#116728

Purpose

Lentiviral expression of CTCF

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CTCF (CTCF Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC476 - pAAV c-fos eYFP

Plasmid

#47907

Purpose

An AAV vector that expresses eYFP under the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

enhanced Yellow Fluorescent Protein

Use

AAV

Tags

Expression

Mutation

Promoter

c-fos

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A315T

Plasmid

#133755

Purpose

Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed alanine 315 to threonine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDP085

Plasmid

#198425

Purpose

pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120

Depositor

Markus Schmid

Article

You et al Nat Commun. 2017 May 17;8:15120. doi: 10

Insert

E. coli BirA

Use

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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p102_gRNA_Sa_mClover

Plasmid

#213778

Purpose

Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sa gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

mU6

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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p103_gRNA_Sp_mClover

Plasmid

#213779

Purpose

Cloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sp gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

mU6

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-mCherry-IRES-Cre

Plasmid

#55632

Purpose

Expresses Cre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Inserts

mCherry
Cre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a and Ef1a/IRES

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-3XFLAG-EGFP-OMP25

Plasmid

#83354

Purpose

For tagging mitochondria with Sigma FLAG epitopes

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XFLAG-EGFP-OMP25

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pICH47751

Plasmid

#48002

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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CRY-Gal∆DD (B1013)

Plasmid

#92035

Purpose

Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Fusion of plant CRY2 to Gal4 binding domain (AA1-…

Promoter

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-tdTomato

Plasmid

#44332

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Has Service

AAV5

Insert

tdTomato

Use

AAV; Adeno-associated virus ()

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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