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Showing: 10401 - 10420 of 16584 results

  2. PM-ST1!TA

    Plasmid
    #48653
    Purpose
    Bacterial ST1 crRNA expression: targets ST1 to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol
    Depositor
    George Church
    Article
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Insert
    Bacterial ST1 crRNA to prototspacer A
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    J23100 promoter
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48653-plasmid-map-sequence-id-71702

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  6. pALS3-Ma-sfGFP-WT

    Plasmid
    #212119
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212119-plasmid-map-sequence-id-444185

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  7. pET29a-human histone 4-in-One

    Plasmid
    #223237
    Purpose
    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4
    Depositor
    Weixing Zhao
    Inserts
    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    Use
    Tags
    Dual StrepII, His6, TEV protease site, and VSVG
    Expression
    Bacterial
    Mutation
    A103G
    Promoter
    T7
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223237-plasmid-map-sequence-id-443806

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  11. pJP_Ctrl10

    Plasmid
    #219672
    Purpose
    Contains PT3lacO promoter (regulated by blue light and LacI) controlling the expression of mCherry. LacI is constitutively produced by pR promoter.
    Depositor
    Yolanda Schaerli
    Article
    Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:
    Inserts
    mCherry
    lacI
    Use
    Tags
    Expression
    Mutation
    Promoter
    PT3lacO and pR
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    219672-plasmid-map-sequence-id-437024

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  13. pBB328

    Plasmid
    #221533
    Purpose
    Plasmid for the introduction of Mariner transposon that inserts deoxyviolacein cassette behind indigenous promoters in target bacterial strain. Plasmid has tetracycline marker for selection.
    Depositor
    Brett Barney
    Inserts
    vioABCE
    Mariner transposase and transposon backbone from pBB298 (originally from pEB001)
    Tetracycline antibiotic cassette from pRK415
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None, contains RBS site
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    221533-plasmid-map-sequence-id-442457

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  14. pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

    Plasmid
    #208628
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 1~41, other residues not included
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208628-plasmid-map-sequence-id-433639

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  15. G555

    Plasmid
    #216483
    Purpose
    Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.
    Depositor
    Christophe Danelon
    Insert
    plsB, plsC, cdsA, pssA
    Use
    Cell-free expression
    Tags
    Expression
    Mutation
    Promoter
    T7 for each gene
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    216483-plasmid-map-sequence-id-430343

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Showing: 10401 - 10420 of 16584 results