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Plasmid#221426PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertTMEM144 (TMEM144 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Sensor_P2A_SLC2A4
Plasmid#221429PurposeGateway entry clone encoding a polyprotein that is cleaved to FRET glucose sensor and SLC2A4 proteinsDepositorInsertSensor_P2A_SLC2A4 (SLC2A4 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_mNeonGreen_FLAG_C
Plasmid#218073PurposeRESOLUTE cloning plasmidDepositorTypeEmpty backboneUseLentiviralTagsFLAG-mNeonGreen-V5ExpressionMutationPromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE ∆238-245
Plasmid#139318PurposeGST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
UseTagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot1iso1_1093-1317-K1208AH1212AK1218A_T
Plasmid#147574PurposeBacterial Expression of HsNot1iso1_1093-1317-K1208AH1212AK1218ADepositorInsertHsNot1iso1_1093-1317-K1208AH1212AK1218A (CNOT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-DrCavin1a
Plasmid#194291PurposeMultisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXMDepositorInsertcavin1a (cavin1a Zebrafish)
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUBI-RZ-Cas12j2
Plasmid#189781PurposeCas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processingDepositorInsertgRNA cloning site
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT079
Plasmid#180509PurposePlasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
DpbCasX
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT077
Plasmid#180511PurposePlasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacersDepositorInsertsCasX sgRNAv2
PlmCasX
UseCRISPRTags2x FLAG and SV40 NLSExpressionMammalianMutationPromoterCAG and U6Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCheck Cdk2ap1ΔN (MT2B2)
Plasmid#178029Purposeexpression vector to test translation efficiency by luciferase reporter.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseLuciferase; NonviralTagsLuciferaseExpressionMammalianMutationPromoterCloned inAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Psicheck Cdk2ap1Exon1
Plasmid#178028Purposeexpression vector to test translation efficiency by luciferase reporter.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseLuciferase; NonviralTagsLuciferaseExpressionMammalianMutationPromoterCloned inAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 4
Plasmid#175815PurposepENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 4 (AT1G09270) without stop codon for C-terminal epitope tagsDepositorInsertIMPORTIN ALPHA ISOFORM 4 (IMPA-4 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationPromoternoneAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 9
Plasmid#175817PurposepENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 9 (AT5G03070.1) without stop codon for C-terminal epitope tagsDepositorInsertIMPORTIN ALPHA ISOFORM 9 (IMPA-9 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationPromoternoneAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265C
Plasmid#164715PurposeGateway entry clone (attL1 & attR5) hAID-CBE_V01 for C-T base editingDepositorInserthAID-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible haid-cbe_v01 entry clo…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E1
Plasmid#164718PurposeGateway entry clone (attL1 & attR5) A3A-CBE_V01 for C-T base editingDepositorInsertA3A-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible a3a-cbe_v01 entry cloneTagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E3
Plasmid#164720PurposeGateway entry clone (attL1 & attR5) eA3A-CBE_V01 for C-T base editingDepositorInserteA3A-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible ea3a-cbe_v01 entry clo…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
BE3-YE1
Plasmid#132943PurposeGene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.DepositorInsertBE3-(W90Y-R126E)
UseCRISPR; Base editorTagsExpressionMammalianMutationBE3-(W90Y-R126E)PromoterAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA
Plasmid#131757PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expressionDepositorInsertTRE#2 Sp/xCas9 gRNA
UseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterhU6Available sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA
Plasmid#131756PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expressionDepositorInsertTRE#1 Sp/xCas9 gRNA
UseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterhU6Available sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA
Plasmid#131755PurposepMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expressionDepositorInsertTRE#1 SaCas9 gRNA
UseSynthetic Biology; Pmagic gateway entry plasmidTagsExpressionMutationPromoterhU6Available sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only