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PB-3xCTCF-TetO

Plasmid

#213023

Purpose

piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

3xCTCF-TetO

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

Expression

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-NUbo-E3(48)-P2A-3xFLAG-Ubc7

Plasmid

#212804

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-P2A-3xFLAG-Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-E3(48)-P2A-3xFLAG-Ubc7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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EXT1- D164A-pLJC2-3XFLAG

Plasmid

#215828

Purpose

Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 Mutant (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-mCherry-NAP1 (WT)

Plasmid

#198036

Purpose

Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Use

Tags

GST-TEV and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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LV-hGFAP-DLX2-ires-GFP

Plasmid

#213602

Purpose

Expression of human DLX2 and GFP under the human GFAP ABC1D promoter

Depositor

Chun-Li Zhang

Article

Zhang et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

DLX2 (DLX2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(48)-NLS-VSV

Plasmid

#212746

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(63)-NLS-VSV

Plasmid

#212756

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NLS-VSV and NUbo

Expression

Yeast

Mutation

Promoter

Tet

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AtSLAC1 WT

Plasmid

#212946

Purpose

Vector used for expression of AtSLAC1 WT in mammalian cell

Depositor

Sangho Lee

Article

Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

Insert

Slow anion channel-associated 1 (OZS1 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGMC00036 (aka pMC0223)

Plasmid

#212659

Purpose

Guide RNA targeting SERPINB3

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

SERBPINB3 guide RNA (SERPINB3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R)-GFP

Plasmid

#212793

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R)-GFP

Use

Integrative vector

Tags

Expression

Yeast

Mutation

CUb(K48R)

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K63R)-GFP

Plasmid

#212794

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K63R)-GFP

Use

Integrative vector

Tags

Expression

Yeast

Mutation

CUb(K63R)

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

Plasmid

#212795

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R/K63R)-GFP

Use

Integrative vector

Tags

Expression

Yeast

Mutation

CUb(K48R/K63R)

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-CUP1-NUbo-E3(63) I227A-VSV

Plasmid

#212757

Purpose

Copper-inducible expression of NUbo-E3(63) I227A-VSV in budding yeast, reduced activity of E3(63)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63) I227A

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Pib1(I227A)

Promoter

CUP1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-E3(48) aa24-150-VSV

Plasmid

#212747

Purpose

Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48) aa24-150

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Cue1 ΔU7BD

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1)-CUbo-mCherry-VSV

Plasmid

#212740

Purpose

Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-mCherry-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-myc-E3(1)-CUbo-mCherry-VSV

Plasmid

#212739

Purpose

Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-mCherry-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

Plasmid

#212738

Purpose

Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

HOIP(C885A)

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-myc-E3(1)-CUbo-NLS-VSV

Plasmid

#212737

Purpose

Consitutive expression of myc-E3(1)-CUbo-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGMC00033 (aka pMC0224)

Plasmid

#210750

Purpose

Guide RNA targeting SERPINB3

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

SERBPINB3 guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RUNX3(13))-PGKpuro2ABFP-W

Plasmid

#208552

Purpose

Lentiviral vector expressing gRNA targeting human RUNX3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX3(13) (RUNX3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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