Purpose
Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
Inserts
WWE domain (R163A) with C-terminal nLuc
WWE domain with C-terminal cLuc
Use
Lentiviral
Tags
FLAG, HA, Split luciferase (cLuc), Split lucifera…
Expression
Mammalian
Mutation
Arg163Ala
Available since
Sept. 14, 2022
Availability
Academic Institutions and Nonprofits only