Addgene

We narrowed to 3,013 results for: actin

Showing: 1021 - 1040 of 3013 results

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pRK5 Myc-tagged PSK (K57A)

Plasmid

#197150

Purpose

Expression of kinase-deficient MYC-tagged PSK1-alpha (K57A) / TAOK2 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Insert

TAOK2 (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2

Plasmid

#196534

Purpose

Expression of GST-Eps8L2-FL

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2 (EPS8L2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 promoter; lac UV5 promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_1-183

Plasmid

#196535

Purpose

Expression of GST-Eps8L2_1-183

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-183 (EPS8L2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 promoter; lac UV5 promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_181-299

Plasmid

#196536

Purpose

Expression of GST-Eps8L2_181-299

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_181-299 (EPS8L2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 promoter; lac UV5 promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_544-715

Plasmid

#196540

Purpose

Expression of GST-Eps8L2_544-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_544-715 (EPS8L2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 promoter; lac UV5 promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5mycGW-Eps8L2_529-715

Plasmid

#196543

Purpose

Expression of myc-Eps8L2_529-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-353)GCN4-L-GFP

Plasmid

#193714

Purpose

Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-353)-GCN4-L-GFP (KIF5B Synthetic, Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-353);

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-353)-GCN4-L-mCherry

Plasmid

#193715

Purpose

Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Synthetic, Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

hsKIF5B(aa1-353); mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR201-Eps8L2_529-715

Plasmid

#196533

Purpose

Entry vector for Gateway with Eps8L2_529-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

CMV promoter; T7 promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_297-370

Plasmid

#196537

Purpose

Expression of GST-Eps8L2_297-370

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_297-370 (EPS8L2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 promoter; lac UV5 promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_367-496

Plasmid

#196538

Purpose

Expression of GST-Eps8L2_367-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_367-496 (EPS8L2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 promoter; lac UV5 promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_494-546

Plasmid

#196539

Purpose

Expression of GST-Eps8L2_494-546

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_494-546 (EPS8L2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 promoter; lac UV5 promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5mycGW-Eps8L2

Plasmid

#196542

Purpose

Expression of myc-Eps8L2-FL

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2 (EPS8L2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)

Plasmid

#174637

Purpose

Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVDfast-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

mVenus-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-365)-VVD52C-mVenus-SSPB(micro)

Plasmid

#174636

Purpose

Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVD52C-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

mVenus-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR N52R (Shank3)

Plasmid

#175255

Purpose

SPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with N52R mutation from SHANK3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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human TLNRD1-4H pET151

Plasmid

#159386

Purpose

Expresses human TLNRD1 4-helix domain (residues 143-273) in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 (TLNRD1 Human)

Use

Tags

his-tag, TEV cleavage site

Expression

Bacterial

Mutation

TLNRD1 4-helix domain (residues 143-273)

Promoter

T7

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA2

Plasmid

#131140

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.27

Plasmid

#99362

Purpose

Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Use

Tags

Expression

Bacterial

Mutation

Glutamic acid 129 to Lysine

Promoter

T7

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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