Addgene

We narrowed to 3,034 results for: aaas

Showing: 1021 - 1040 of 3034 results

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pColE1_sgRNA_T2_AmpR

Plasmid

#119159

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T3_AmpR

Plasmid

#119160

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_T1_AmpR

Plasmid

#119158

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, truncated in the 3' end

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCR-Hyg

Plasmid

#158708

Purpose

For cloning and constitutive expression of crRNAs in mycobacterium

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

crRNA

Use

CRISPR and Synthetic Biology; Mycobacteria expres…

Tags

Expression

Bacterial

Mutation

Promoter

Hsp60

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCR-Zeo

Plasmid

#158709

Purpose

For cloning and constitutive expression of crRNAs in mycobacterium

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

crRNA

Use

CRISPR and Synthetic Biology; Mycobacteria expres…

Tags

Expression

Bacterial

Mutation

Promoter

Hsp60

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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hRIP3 GFP RHIM mut

Plasmid

#41385

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

RHIM mutant (AAAA-459-462)

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01(ΔL1)

Plasmid

#80049

Purpose

Expression of EPN-01 mutated in TSG101 binding site.

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaPTAP)

Use

Tags

Myc

Expression

Mammalian

Mutation

PTAP10AAAA

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-STAT1

Plasmid

#117160

Purpose

Target site ATATCCAGTTCCTTTAGGGCC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AasgRNA3.8

Plasmid

#121958

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA, short version

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTGMP-p16/p19.478

Plasmid

#32717

Depositor

Scott Lowe

Article

Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

Insert

p16/p19

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
HA Raptor mutant 3

Plasmid

#8473

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Use

Tags

ha

Expression

Mammalian

Mutation

313NWIF316-AAAA (N313A, W314A, I315A, F316A)

Promoter

Available since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pUC19-U6-BssgRNA

Plasmid

#121957

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

BsCas12b single chimeric gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJT303_SNR52_sgRNA_Can1-3

Plasmid

#145066

Purpose

Expresses sgRNA targeting Can1-3 site

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

Can1-3 sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-IRF9

Plasmid

#117159

Purpose

Target site AATTATCACAAAGAGGACAGG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-H11r1-2-DD-Cas9

Plasmid

#85978

Purpose

Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

DD-SpCas9

Use

CRISPR

Tags

FKBP12 L106P DD

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF331_2

Plasmid

#135740

Purpose

Encodes gRNA for 3' target of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 gRNA (ZNF331 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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p8390 LentiCRISPRv2 Neo sgNT-2

Plasmid

#221650

Purpose

Expression of non-targeting control sgRNA

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

non-targeting sgNT-2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD2 N-terminal sgRNA

Plasmid

#207098

Purpose

pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TTTTTATCAGAAATCATGAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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53BP1 KO sgRNA

Plasmid

#207104

Purpose

pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGATTCTCAGCCTGAAAGCC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A4

Plasmid

#208193

Purpose

frameshift mistranslation, A4

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Tags

Expression

Bacterial

Mutation

Frameshift insertion 6AAAA

Promoter

Ptet+dnaK P1

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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