Addgene

We narrowed to 1,454 results for: P11

Showing: 1021 - 1040 of 1454 results

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pCB-1

Plasmid

#210387

Purpose

All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

Depositor

Gilles van Wezel

Article

Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

Inserts

dCas9
CymR
sgRNA cassette

Use

CRISPR; Integrative vector for use in streptomyces

Tags

Expression

Mutation

BsaI-flanked RFP cassette inserted in place of sp…

Promoter

SP11 and SP30

Availability

Academic Institutions and Nonprofits only

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pCB-2

Plasmid

#210388

Purpose

All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

Depositor

Gilles van Wezel

Article

Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

Inserts

dCas9
CymR
sgRNA cassette

Use

CRISPR; Integrative vector for use in streptomyces

Tags

Expression

Mutation

BsaI-flanked RFP cassette inserted in place of sp…

Promoter

SP11 and SP30

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB2 CTF-FLAG

Plasmid

#217682

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB2 (BAI2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-911

Promoter

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG

Plasmid

#217715

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB2 (BAI2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-917

Promoter

Availability

Academic Institutions and Nonprofits only

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pGM8

Plasmid

#220166

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TSPO (TSPO Human)

Use

Lentiviral

Tags

GFP11

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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KHBD00343

Plasmid

#34466

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11992 (Rel Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pBabe puro-HA-PIK3CA(H1047R;I848V)

Plasmid

#33253

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and isoleucine…

Promoter

SV40

Availability

Academic Institutions and Nonprofits only
pBabe puro-HA-PIK3CA(H1047R;G320E)

Plasmid

#33254

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and glycine 32…

Promoter

SV40

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shGDF11 #2

Plasmid

#83084

Purpose

Lentiviral shRNA vector for inducible knockdown of human GDF11

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGDF11 (GDF11 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HSPH2

Plasmid

#19454

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 4 (HSPA4 Human)

Use

Tags

Expression

Mammalian

Mutation

mutations A381T, T732A, and an insertion of "…

Promoter

Availability

Academic Institutions and Nonprofits only

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pET151-HIS-TEV-GST-PP-RPN13

Plasmid

#73741

Purpose

Expression of hRPN13 in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN13 (ADRM1 Human)

Use

Tags

6xHIS and GST

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only
pET151-GST-PP-RPN13 (19-132)

Plasmid

#73742

Purpose

Expression of human RPN13 PRU domain in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN13 (ADRM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

PRU domain only (residues 19-132)

Promoter

T7

Availability

Academic Institutions and Nonprofits only
PAN3 gRNA (BRDN0001146404)

Plasmid

#77423

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAN3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAN3 (PAN3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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PAN3 gRNA (BRDN0001146135)

Plasmid

#77424

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAN3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAN3 (PAN3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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PAN3 gRNA (BRDN0001147703)

Plasmid

#77425

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAN3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAN3 (PAN3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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PAN3 gRNA (BRDN0001146176)

Plasmid

#77426

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAN3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAN3 (PAN3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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myc-Med22 pBacPAK8

Plasmid

#15374

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med22 (MED22 Human)

Use

Baculovirus construction

Tags

myc

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pAG75 ARHGEF18 3'UTR mut

Plasmid

#12055

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N1 3'UTR mut (ARHGEF18 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Availability

Academic Institutions and Nonprofits only

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FLAG-MED22

Plasmid

#15423

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED22 (MED22 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Availability

Academic Institutions and Nonprofits only
pET41a-MED22

Plasmid

#15432

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED22 (MED22 Human)

Use

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Promoter

Availability

Academic Institutions and Nonprofits only