-
Plasmid#119775PurposeExpression of a target sgRNA in potatoDepositorInsertAtU6-SmR-sgRNA
UseTagsExpressionPlantMutationPromoterAtU6Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-lynD3cpV
Plasmid#37472DepositorInsertD3cpv second generation cameleon (calcium sensor)
UseTagsthe myristoylation and palmitoyla- tion sequence …ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5C6A,P-J23106-geneIII
Plasmid#80860PurposePhagemid (encodes evolved cI5C6A,P and M13 gene III)DepositorInsertsM13 gene III
cI5C6A,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFM689
Plasmid#74635Purposealias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection markerDepositorInsertsUseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-GSK3B_IDG-K
Plasmid#158166PurposeGateway destination clone of V5-GSK3B (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-GSK3B (GSK3B Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIM690
Plasmid#74638Purposealias pFA6a-yTagRFP-T-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and hygromycin selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
3265 pBS Mcl-1
Plasmid#8763DepositorInsertMcl-1 (Mcl1 Mouse)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
-
pMSSM97: pCMV-HA-NLS-SpuFz1-E498R
Plasmid#205275PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-E498RDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationE498RPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM105: pCMV-HA-NLS-SpuFz1-T513K
Plasmid#205276PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513KDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationT513KPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM58: pCMV-HA-NLS-SpuFz1-D487K
Plasmid#205274PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487KDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationD487KPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM59: pCMV-HA-NLS-SpuFz1-D300R
Plasmid#205272PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300RDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationD300RPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ273: pRS426-His-MBP-GtFz1_PSP1
Plasmid#205258PurposeS. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)DepositorInsertGtFz1
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ127: pRS426-His-MBP-NlovFz2_PSP1
Plasmid#205257PurposeS. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)DepositorInsertNlovFz2
UseTags10xHis, MBPExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIM572
Plasmid#74640Purposealias pFA6a-yTagRFP-T-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagRFP-T and G418 selection markerDepositorInsertyTagRFP-T
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMEH361 Hspa8 MS2 HA
Plasmid#168218PurposeMammalian expression of heat shock 70 kDa protein 8 fused to the MS2 dimer and C-terminal HA tagDepositorInsertHspa8
UseTagsHA and MS2 dimerExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
RECQL5 (RECQL5A-c011)
Plasmid#98231PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRECQL5A (RECQL5 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-ESCRT 0
Plasmid#21499DepositorInsertpFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Mouse, Human)
UseTagsGST and His-MBPExpressionInsectMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
-
pLITMUS-rpoN-cI5C6A-J23106-geneIII
Plasmid#80905PurposePhagemid (encodes cI5C6A and M13 gene III)DepositorInsertsM13 gene III
cI5C6A
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only