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Plasmid#108282PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB-
Plasmid#62540PurposeCloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate CloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSADdeltaG-FLPo-DsRedX
Plasmid#32650DepositorInsertsFLPo
DsRedX
UseRabies virusTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMM705
Plasmid#68892PurposeIPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
UseTagsExpressionMutationPromoterAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2162
Plasmid#110136PurposeEpidermal Mitochondrial-tagged Florescent Calcium SensorDepositorInsertmito::GCaMP3
UseTagsmito::GCaMP3ExpressionWormMutationPromoterPcol-19Available sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPV033
Plasmid#178359PurposeTargeted gene knock-in plasmid for Schizophyllum communeDepositorInsertsLeft flank
nour_cassette
Right flank
UseFungal homologous recombination plasmidTagsExpressionMutationPromoterS. commune gpd and n.a.Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJKR-L-ttgR
Plasmid#62566PurposeFlavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-N-P-M-EGFP-SynPhRFP-L
Plasmid#52483PurposeExpresses EGFP and Synaptophysin-TagRFP-TDepositorInsertsEGFP
Synaptophysin-TagRFP-T
UseRabies virusTagsTagRFP-TExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-I70V-EsaI
Plasmid#47661PurposeesaI gene downstream of esaR-a208g in pAC-EsaR-I70V (CmR)DepositorInsertesaI-term
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G-EsaI
Plasmid#47662PurposeesaI gene downstream of esaR-a272g in pAC-EsaR-D91G (CmR)DepositorInsertesaI-term
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHnCB
Plasmid#52064Purposeexpresses nine genes from the H. neapolitanus carboxysome operon; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
hOCT1-miRFP
Plasmid#188995PurposeA mammalian expression plasmid encoding miRFP-tagged human OCT1DepositorInserthOCT1 (SLC22A1 Human)
UseTagsmiRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSADdeltaG-GCaMP3-DsRedX
Plasmid#32645DepositorInsertsGCaMP3
DsRedX
UseRabies virusTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-H-RAN-RFP6
Plasmid#106426PurposeH-RAN-RFP6 (RANbody RFP6::Sm-HAtag)DepositorInsertH-RAN-RFP6
UseTagsHA, His, and Multiple HAExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKB234.2
Plasmid#170023PurposeEncodes LacI-controlled expression of surface-displayed hBD3 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
hBD3 Δ alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceDec. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-TetR-cryaa-YFP
Plasmid#73202PurposeBAC targeting cassette for pTARBAC2 with tetR selection marker and lens-specific transgenesis marker CitrineDepositorInsertYFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSMART-2,3-BDO1
Plasmid#65816PurposeNADH Dependent 2,3-BDO Production Construct, Low Phosphate Dependent E. coli ExpressionDepositorInsertsbudA: α-acetolactate decarboxylase
budB: acetolactate synthase
budC: acetoin reductase
UseTagsExpressionBacterialMutationPromoterwaaHp from E. coli as part of operon with budA an…Available sinceJuly 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWR
Plasmid#112905PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]
Plasmid#124069PurposeCRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)DepositorInserteb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40
UseCRISPR and Cre/LoxTagsExpressionInsectMutationPromoterbhsp68Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-338
Plasmid#68795PurposeSingle construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry markerDepositorInsertsC-Terminal Sensor 1 V1
mCherry
ZF9 Op x6
EGFP
N-Terminal Sensor 1 V2
UseTagsIRES, NLS, flag, and nlsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only