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We narrowed to 52,188 results for: cloning

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pDONR221_SLC24A2

Plasmid

#131966

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC24A2 (SLC24A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35A4

Plasmid

#132123

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A4 (SLC35A4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35B1

Plasmid

#132099

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35B1 (SLC35B1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC2A10

Plasmid

#132091

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A10 (SLC2A10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35E1

Plasmid

#132098

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35E1 (SLC35E1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC36A4

Plasmid

#132143

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC36A4 (SLC36A4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35D3

Plasmid

#132110

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35D3 (SLC35D3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE

Plasmid

#139092

Purpose

Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

dxCas9 3.7-VPR

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC17A4

Plasmid

#131924

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC17A4 (SLC17A4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCAT526

Plasmid

#180513

Purpose

Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv1
PlmCasX-2A-mNeonGreen

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC4A8

Plasmid

#132300

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A8 (SLC4A8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35F5

Plasmid

#132085

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35F5 (SLC35F5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Moe-pDONR221

Plasmid

#124239

Purpose

Gateway entry clone of Drosophila Moesin cDNA encoding the D isoform.

Depositor

Peter Vilmos

Article

Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

Insert

Drosophila Moesin (Moe Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

Plasmid

#86195

Purpose

U6 based expression of N meningitidis sgRNA

Depositor

Scot Wolfe

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

nmCas9 sgRNA cloning cassete

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC2A7

Plasmid

#132066

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A7 (SLC2A7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC35A3

Plasmid

#132135

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A3 (SLC35A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pUCIDT-attL1-Worm ABeta-attR5

Plasmid

#160437

Purpose

Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Homo sapiens amyloid beta precursor protein (APP Nematode, Human)

Use

Tags

Cleavage signal

Expression

Bacterial

Mutation

Codon-optimized for expression in C. elegans

Promoter

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SNORD27h_2ntmut

Plasmid

#73067

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (E2F7 Human)

Use

Tags

no tag

Expression

Mammalian

Mutation

2 nt mutation in AS box that binds E2F7 pre-mRNA

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pDONR223-GOLPH3

Plasmid

#21688

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

last nucleotide of stop codon removed for c-termi…

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC25A52

Plasmid

#132031

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A52 (SLC25A52 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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