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Plasmid#132285PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSPNS3 (SPNS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
BE3-hA3A-R128A
Plasmid#132944PurposeGene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.DepositorInsertBE3-hA3A(R128A)
UseCRISPR; Base editorTagsExpressionMammalianMutationBE3-hA3A(R128A)PromoterAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC9B1
Plasmid#132223PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC9B1 (NHEDC1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC2A4
Plasmid#132102PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC2A4 (SLC2A4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC9A5
Plasmid#132163PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC9A5 (SLC9A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-AviMta2-3xFiBsd
Plasmid#140964Purposefull-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cellsDepositorInsertmetastasis-associated gene family, member 2 (Mta2 Mouse)
UseTags3xFLAG, Avi, and TEVExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265G2
Plasmid#164723PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editingDepositorInsertA3A(Y130F)-ziSpyMac(D10A)-UGI)
UseCRISPR; Gateway compatible a3a/y130f-ispymaccas9-…TagsExpressionPlantMutationPromoterAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A11
Plasmid#131963PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A11 (SLC22A11 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC41A3
Plasmid#132166PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC41A3 (SLC41A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC12A9
Plasmid#131897PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC12A9 (SLC12A9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE
Plasmid#139091PurposeExpresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertdSpCas9-NG-VPR
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…PromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC45A4
Plasmid#132179PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC45A4 (SLC45A4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYLsgRNA-OsU3m/LacZ
Plasmid#66192Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterOsU3m (SpeI site destroyed)Available sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
BE3-hA3A-Y130F
Plasmid#132945PurposeGene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.DepositorInsertBE3-hA3A(Y130F)
UseCRISPR; Base editorTagsExpressionMammalianMutationBE3-hA3A(Y130F)PromoterAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A48
Plasmid#131995PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A48 (SLC25A48 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pelB-MBP-RevA-ss
Plasmid#137064PurposeE. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAF07416.1 (revA Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationlacks the RevA signal peptide and 5 residues (CKA…PromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC15A2
Plasmid#131886PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC15A2 (SLC15A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pelB-MBP-P66-ss
Plasmid#137045PurposeE. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66949.1 (p66 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P66: lacks the P66 signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC10A4
Plasmid#131943PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC10A4 (SLC10A4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDest-1.7kbfabp6-eGFP-pA-CrymCherry
Plasmid#159088PurposeLR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA insertsDepositorInsertsUseFluorescent reporterTagsExpressionMutationPromoter1.7kb fabp6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only