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pFS16 Pmyo-3 NPP-9 mChBioFLAG

Plasmid

#45099

Depositor

Steven Henikoff

Article

Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

Inserts

npp-9 (npp-9 Nematode)
unc-119 (unc-119 Nematode)

Tags

BLRP, FLAG, and mCherry

Expression

Worm

Promoter

myo-3 and unc-119

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAV116

Plasmid

#63183

Purpose

pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12g1

Plasmid

#120879

Purpose

Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12g1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Promoter

lac

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfCCK-0.5k-Venus-WPRE

Plasmid

#156392

Purpose

Expresses Venus under CCK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cluc-RMA

Plasmid

#189624

Purpose

Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

Cypridina luciferase fused to Fc

Use

AAV and Luciferase

Tags

Cluc and IgG1 Fc

Expression

Mammalian

Promoter

hSyn

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPENK-0.9k-Venus-WPRE

Plasmid

#156401

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfSST-0.3k-Venus-WPRE

Plasmid

#156393

Purpose

Expresses Venus under SST gene promoter

Depositor

Shuji Kaneko

Article

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1 243-450

Plasmid

#24392

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 243-450.

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA4-Beclin1 151-241

Plasmid

#24390

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 151-241.

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag mHMGB2

Plasmid

#31610

Depositor

Yasuhiko Kawakami

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

Insert

HMGB2 (Hmgb2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pCS2+ Zf GFP-vclb

Plasmid

#185411

Purpose

GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin b (vclb Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHLP712

Plasmid

#203741

Purpose

Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Mutation

Codon 74 changed from Phe to Ala (F74A)

Promoter

ACT1 (C. albicans)

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAV125

Plasmid

#63191

Purpose

pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dPlcR(K30/32L)

Plasmid

#181833

Purpose

Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dPlcR (K30L,K32L)

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52546

Purpose

Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf GFP-vcla

Plasmid

#185410

Purpose

GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin a (vcla Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSW610 - GR-LhG4_BD

Plasmid

#115992

Purpose

entry vector for GreenGate cloning method, CDS, with adaptors for B and D modules

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

GR-LhG4_BD

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR

Plasmid

#174883

Purpose

Targeting vector for generating Pou5f1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Pou5f1

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68

Plasmid

#153527

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#68

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#68

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSB117 - pL2_pSB90_fLUC-I_V2

Plasmid

#123192

Purpose

binary plant vector for transient TYLCV V2 and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::V2::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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