Addgene

We narrowed to 11,165 results for: cel.2

Showing: 10241 - 10260 of 11165 results

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AAV_Actb MMEJ donor

Plasmid

#97315

Purpose

MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb MMEJ donor

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Efs_hSpCas9_NLS_FLAG-SV40

Plasmid

#97307

Purpose

AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

AAV and Mouse Targeting

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-221-PGK-GFP-Blast

Plasmid

#31519

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-221 (Mir221 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pSpCas9-Puro HLAG-2A-sgRNA

Plasmid

#182551

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2A-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9-Puro HLAG-2B-sgRNA

Plasmid

#182552

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2B-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Sox2 HMEJ donor

Plasmid

#97322

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Sox2. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Sox2 HMEJ donor

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Nanog HMEJ donor

Plasmid

#97321

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Nanog HMEJ donor

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Cdx2 HMEJ donor

Plasmid

#97319

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Cdx2 HMEJ donor

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-EglN2 wt-pBabe-Puro

Plasmid

#22704

Depositor

William Kaelin

Article

Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

Insert

Egg laying Nine - 2 (EglN2) (EGLN2 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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AAV_Dbh HMEJ donor

Plasmid

#97320

Purpose

HMEJ donor for fusing a p2A-mCherry-WPRE reporter to mouse Dbh. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Dbh HMEJ donor

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-221SM-PGK-GFP-Blast

Plasmid

#31520

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-221 (Mir221 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pIRES2-eGFP:HA-mTREK-1(S131C)

Plasmid

#224774

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S131C

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (C159A/C219A)

Plasmid

#224751

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

C159A/C219A

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/K271Q)

Plasmid

#224750

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/K271Q)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C / K271Q

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/G171F)

Plasmid

#224749

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/G171F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C / G171F

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/A286F)

Plasmid

#224748

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/A286F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C / A286F

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-Prf1-ires-mCherry

Plasmid

#209073

Purpose

Targeting vector for the mouse Prf1 locus to correct prf1 gene

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

Prf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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CS-TRE-BMI1-Ubc-rtTA

Plasmid

#198191

Purpose

Generation of Expandable megakaryocyte cell lines from human iPS cells

Depositor

Hiromitsu Nakauchi

Article

Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

Insert

hBMI1 (BMI1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTet-O-Ngn2-puro

Plasmid

#52047

Purpose

3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

Depositor

Marius Wernig

Article

Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

Insert

Ngn2 (Neurog2 Mouse)

Use

Lentiviral

Tags

T2A-and puromycin resistant cassette

Expression

Mammalian

Mutation

Promoter

tet-on

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

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pJR85

Plasmid

#140095

Purpose

Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.

Depositor

Jonathan Weissman

Article

Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

Insert

sgRNA with cs1 in stem loop 2 of the constant region

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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