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Plasmid#135817PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertcysteine peptidase C (CPC)
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
D10_Ld_292250
Plasmid#135826PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertC-8 sterol isomerase-like protein
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
D17_Ld_090870
Plasmid#135833PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInserthypothetical protein, conserved
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
D8_Ld_340400
Plasmid#135824PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInserthypothetical protein, unknown function
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PnGW
Plasmid#133355PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDR-nfatc1_sgRNA2
Plasmid#132978Purposenfatc1 sgRNA targeting to "5'-GTGGGAGCTCCATTGGATCG-3" in pDR274DepositorInsertnfatc1 sgRNA2 target sequence
UseCRISPR; Sgrna generation vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
K8.1Pr-pGL4.16-OriLyt
Plasmid#131038PurposeFirefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replicationDepositorInsertKSHV Lytic Origin of replication
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG
Plasmid#130684PurposeExpresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationD453_F489del, E653_G1960delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG
Plasmid#130683PurposeExpresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationD409_E452del, E653_G1960delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.007
Plasmid#119671PurposeLevel 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.DepositorInsertPcpc560-Dx2
UseSynthetic BiologyTagsExpressionMutationbp 89 T to C, bp 187 C to A, Duplicated downstrea…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.006
Plasmid#119670PurposeLevel 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.DepositorInsertPcpc560-Ux2
UseSynthetic BiologyTagsExpressionMutationbp 89 T to C, bp 187 C to A, Duplicated upstream …PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pAPM-D4 miR30-SUV39h2 ts1
Plasmid#115883PurposeSUV39h2 knockdownDepositorInsertSUV39h2 shRNA (SUV39H2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZA-YFP-R5
Plasmid#106257PurposeExpresses Venus YFP fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertYellow fluorescent protein
UseTagsAdded silaffin R5 peptide to C terminus of YFP af…ExpressionBacterialMutationPromoterpL-TetOAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21d_SNORD78_probe
Plasmid#73072Purposeantisense probe for detection of human SNORD78 (C/D box snoRNA U78)DepositorInsertSNORD78 (SNORD78 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS
Plasmid#107300PurposeExpresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cellsDepositorInsertTruncated SpCas9 (aa 574-1368) fused to FKBP
UseCRISPRTags3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …ExpressionMammalianMutationTruncated SpCas9 (aa 574-1368) with R1335K mutati…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS
Plasmid#107299PurposeExpresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cellsDepositorInsertTruncated SpCas9 (aa 574-1368) fused to FKBP
UseCRISPRTags3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …ExpressionMammalianMutationTruncated SpCas9 (aa 574-1368) with R1335K mutati…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAL4DBD-SuUR f.l
Plasmid#112995PurposeP element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genomeDepositorInsertSuUR (SuUR Fly)
UseP insertion vectorTagsExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-F219L (JBEI-7186)
Plasmid#110151PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant geneDepositorInsertGGR
UseTagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-F219L mutant genePromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys
Plasmid#110541PurposeBacterial protein of E.coli DHFR C85A/C152S mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationC85 mutated to A85, C152 mutated to S152PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only