-
Plasmid#106151PurposeEasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistanceDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT371
Plasmid#125612PurposeExpresses CDA1-BE4max-NG in mammalian cellsDepositorInsertCDA1-BE4max-NG
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i1
Plasmid#120882PurposeExpresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.DepositorInsertCas12i1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1354
Plasmid#69490PurposeGly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology armsDepositorInsertGSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
UseCRISPR; Template to generate cassettes for -media…TagsExpressionMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-mCherry
Plasmid#79509PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDSDepositorInsertmCherry RFP
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CASI-Cas9C
Plasmid#80932PurposeExpresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter.DepositorInsertCas9C
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA(MS2)-puro-barcode
Plasmid#89493PurposeCloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seqDepositorInsertsgRNA (with MS2 loop)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-TIGHT-EGFP-backward donor
Plasmid#22077DepositorInsertTETO-eGFP
UseTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pT7-eGFP
Plasmid#32552DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterpT7Available sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCfB6638
Plasmid#106165PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistanceDepositorInsertunknown
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-TCF7
Plasmid#79498PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDSDepositorInsertTCF7 (TCF7 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-NC
Plasmid#30226DepositorInsertrhVitronectin with N and C terminus cleavages (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 399-478, 20-62PromoterAvailable sinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12g1
Plasmid#120879PurposeExpresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.DepositorInsertCas12g1
UseCRISPRTagsmH6ExpressionBacterialMutationPromoterlacAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV MN (eGFP)
Plasmid#89932PurposeExpresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.DepositorInsertMN
UseTagsHAExpressionMammalianMutationM.SssI residues 2-272 fragmentPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_minSV40-sfGFP-CMV_dsRed
Plasmid#117400PurposesfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP_LD 0_TEV
Plasmid#58877PurposeMESA protease chain with Rapamycin FKBP ectodomain and no linker domainDepositorInsertMESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
UseAAVTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
BiP 2: C. elegans BiP (hsp-4)-GFP
Plasmid#21896DepositorInsertBiP (hsp-4 Nematode)
UseTagsGFPExpressionWormMutationFusion between the GFP and hsp-4 takes place at A…PromoterAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa-VPR mini.
Plasmid#99685PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoterDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_EcSSPB-nano_X
Plasmid#131212PurposeExpresses EcSSPB-nano (Escherichia coli) followed by restriction sitesDepositorInsertEcSSPB-nano
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBDC
Plasmid#135188PurposeIntegration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.DepositorInsertCmR
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromotercatAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only