Addgene

We narrowed to 42,392 results for: eng

Showing: 10201 - 10220 of 42392 results

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pconst #4-RFP

Plasmid

#81078

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Jay Keasling, Paul Adams

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

Promoter

Constitutive Promoter - BBa_J23107

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pTomo-AurA

Plasmid

#26293

Depositor

Inder Verma

Article

Marumoto et al Nat Med. 2009 Jan . 15(1):110-6.

Insert

aurora kinase A (AURKA Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3aHA

Plasmid

#18030

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3a (Wnt3a Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

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mPapaya1-PDHA1-N-10

Plasmid

#56356

Purpose

Localization: Mitochondria, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

PDHA1 (PDHA1 Human)

Use

Tags

mPapaya1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L1L2

Plasmid

#113735

Purpose

Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Tags

Expression

Mutation

Promoter

P. patens U6 promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher EQ

Plasmid

#35045

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher EQ

Use

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Promoter

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pTD-NTwinStrep_Sm

Plasmid

#45937

Purpose

use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacIq-Ptrc

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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mOtop2_pGEMHE

Plasmid

#114675

Purpose

This construct was used to express mouse Otop2 in Xenopus laevis oocytes

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 2 (Otop2 Mouse)

Use

For generating the mrna, which can highly express…

Tags

Expression

Mutation

Promoter

T7

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBT29-splitD

Plasmid

#122599

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-let7 sponge

Plasmid

#35664

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

U6 let-7sponge

Use

Tags

Expression

Mutation

Promoter

U6

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR221-CAG.Pro

Plasmid

#79490

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CAG Promoter

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

CAG Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pPAiRFP2-N1

Plasmid

#44273

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

Insert

PAiRFP2

Use

Tags

Expression

Mammalian

Mutation

K69R/R83K/G120D/A123T/M163L/Q168E/R220P/S243N/V24…

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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SNV-gfp-IRES-MCS

Plasmid

#53122

Purpose

Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

Depositor

Jeanette Hyer

Article

Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

Type

Empty backbone

Use

Retroviral; For expression in chicken cells

Tags

EGFP-IRES

Expression

Mutation

Promoter

SNV (spleen necrosis virus)

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12c2

Plasmid

#120873

Purpose

Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c2

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBT29-splitC

Plasmid

#122598

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTRANS_213

Plasmid

#91112

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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p426 46Q GPD

Plasmid

#1182

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 46Q (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
mEos4a-C1

Plasmid

#54810

Purpose

Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Use

Tags

mEos4a

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSiMPlk_N + pSiMPlk_C

Plasmid

#134310

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Article

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

kanR_gp41-1 N-intein + kanR_gp41-1 C-intein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-G9adSET

Plasmid

#41722

Purpose

Retroviral expression of human G9a mutant in mammalian cells

Depositor

Kai Ge

Article

Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

Insert

G9adset (EHMT2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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