Addgene

205,743 results

Showing: 10201 - 10220 of 205743 results

Results per page:

Sort results by:

pEF5B-FRT-control-APEX

Plasmid

#73187

Purpose

Expresses "control"-APEX (cytosolic) in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

control-APEX (Nphp3 Mouse, Glycine max)

Use

Tags

internal GFP tag

Expression

Mammalian

Mutation

changed glycine 2 to alanine

Promoter

EF1alpha

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAdEasy-2

Plasmid

#16401

Purpose

Adenoviral vector for use in AdEasy System, for use with large inserts.

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
WZLneo-Cre

Plasmid

#34568

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Cre

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe puro c-met WT

Plasmid

#17493

Depositor

Joan Brugge

Article

Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

Insert

c-Met (Met Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
p40PX-EGFP

Plasmid

#19010

Depositor

Michael Yaffe

Article

Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Insert

p40phox (NCF4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa1-148 (PX domains)

Promoter

CMV

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-His-AlaRS

Plasmid

#124103

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Alanyl-tRNA ligase (alaS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MED19

Plasmid

#15420

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pMRX-IZ-ANXA7-mRuby3

Plasmid

#184893

Purpose

Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA7 (ANXA7 Human)

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.TBG mCherry-LDTS-LIRmut

Plasmid

#189003

Purpose

lipophagy-inducing adaptor protein, AAV, negative control

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-LIRmut, mCherry

Use

AAV

Tags

HA

Expression

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK7-HA-S6K1-F5A-E389-R3A

Plasmid

#8991

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

F5A, T389E, R3A (C-terminal RSPRR motif to ASPAA)

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneoGFP-BMI1

Plasmid

#128328

Purpose

Mammalian expression vector of GFP-tagged human BMI1

Depositor

Yutaka Hata

Article

Hossain et al Mol Cell Biol. 2018 Aug 15;38(17). pii:

Insert

BMI1 (BMI1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBI-CRY2PHR-VPR

Plasmid

#192606

Purpose

Plasmid for constitutive expression of CRY2PHR-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

CRY2PHR-VPR

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN-LexAOx4-mNeonGreen

Plasmid

#192604

Purpose

Vector for inducible mNeonGreen expression with 4x LexA operator (LexAO)

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

mNeonGreen

Use

Unspecified

Tags

Expression

Mutation

Promoter

Minimal promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pScarlessHD-sfGFP-DsRed

Plasmid

#80811

Purpose

Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Kate O'Connor-Giles

Insert

sfGFP-piggyBac-DsRed

Use

Unspecified

Tags

sfGFP

Expression

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Nsp13

Plasmid

#157712

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp13 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1

Plasmid

#58434

Purpose

Induces Citrine expression from a promoter containing 4 lexA boxes in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

CitrineA206K

Use

Tags

Expression

Yeast

Mutation

Promoter

insul-(lexA-box)4-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
PM-RA

Plasmid

#211705

Purpose

Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cells

Depositor

Yasunori Saheki

Article

Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

Insert

PM-targetted RA (Lck Synthetic)

Use

Tags

RA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Use

Tags

10xHis-MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV2-hSyn-G-Flamp1-mut

Plasmid

#188570

Purpose

control probe of G-Flamp1, insensitive to cAMP

Depositor

Jun Chu

Article

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1-mut

Use

AAV

Tags

His tag

Expression

Mutation

Promoter

hSyn

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-HLA-cmyc-optiT1R3 a21-852

Plasmid

#113948

Purpose

mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R3 (TAS1R3 Human)

Use

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 21-852

Promoter

CMV

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge