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Plasmid#103031PurposeLentiviral expression of mRuby2-tagged MRLC with puromycin selectionDepositorInsertMRLC1 (MYL9 Human)
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HOXB13_#2
Plasmid#70094PurposeExpression of shRNA to human HOXB13, puromycin selectionDepositorInsertHOXB13 (HOXB13 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HOXB13_#1
Plasmid#70093PurposeExpression of shRNA to human HOXB13, puromycin selectionDepositorInsertHOXB13 (HOXB13 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Dnmt3b1-Poly(A)-PuroR
Plasmid#65556PurposePlasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selectionDepositorInsertDnmt3b (Dnmt3b Mouse)
UseMouse Targeting; Bxb1TagsmCherryExpressionMammalianMutationisoform 1PromoterAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-Blast-Puro
Plasmid#167186PurposeLentiviral expression of sgRNA with blasticidin and puromycin resistance genesDepositorInsertBlasticidin S deaminase
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flag-Avi-ires-CHD5
Plasmid#68878PurposeTransient mammalian expression of CHD5 for BirA taggingDepositorInsertCHD5 (CHD5 Human)
UseTagsFLAG/AVIExpressionMammalianMutationPromoterCAGAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK1-MEK5DD
Plasmid#53176PurposeExpresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from puromycin resistance retroviral vectorDepositorInsertMEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)
UseRetroviralTagsHAExpressionMammalianMutationMEK1 (1-201) MEK5(498–1344 with S311D/T315D)PromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgATXN1L-1
Plasmid#74970PurposesgATXN1L-1, puromycin selectionDepositorInsertATXN1L (ATXN1L Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCB320-sgZmat3.51.5
Plasmid#169024PurposeExpresses an sgRNA targeting Zmat3DepositorInsertsgRNA targeting Zmat3, mCherry, Puromycin resistance
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMammalianMutationPromotermU6Available sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCB320-sgZmat3.55.9
Plasmid#169026PurposeExpresses an sgRNA targeting Zmat3DepositorInsertsgRNA targeting Zmat3, mCherry, Puromycin resistance
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMammalianMutationPromotermU6Available sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTBL681 CHYRON4 integration construct
Plasmid#126449PurposeTo integrate the CHYRON4 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON4 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30
Plasmid#163359PurposeLentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.DepositorInsertLy-6G (Ly6g Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterEF1Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-Ly6G-P2A-PuroR-HA-Rluc_miR
Plasmid#163340PurposeRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and an HA-tagged puromycin selection markerDepositorInsertLy-6G (Ly6g Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 (S610L)
Plasmid#31800DepositorInsertGRM3 (GRM3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged serine 610 to leucinePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 (E870K)
Plasmid#31802DepositorInsertGRM3 (GRM3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged glutamic acid 870 to lysinePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgATXN1L-2
Plasmid#74971PurposesgATXN1L-2, puromycin selectionDepositorInsertATXN1L (ATXN1L Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 (E767K)
Plasmid#31801DepositorInsertGRM3 (GRM3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged glutamic acid 767 to lysinePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 (G561E)
Plasmid#31799DepositorInsertGRM3 (GRM3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged glycine 561 to glutamic acidPromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro
Plasmid#211821PurposePiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishmentDepositorInsertSpCas9-ABE8e, tracrRNA and Puromycin resistance gene
UseTagsExpressionMammalianMutationPromoterchicken β-actin promoter, U6 promoter and hPGK pr…Available sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR
Plasmid#211815Purposelentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishmentDepositorInsertSpCas9, tracrRNA and puromycin selection gene
UseLentiviralTagsExpressionMammalianMutationPromoterU1a promoter, U6 promoter, and hPGK promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only