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Plasmid#167510PurposeBroad-Host range expression vector, pUC origin, Tn7 recognition sites, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertVanRAM-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPVanCCAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLO43.3_human ProCas9Flavi
Plasmid#121629PurposeU6-sgRNAdest_CMV-intron_hProCas9Flavi_T2A Mcherry_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCM54
Plasmid#220830PurposeEryA sensor with increased sensitivity due to phosphorylation of EryA by mphA, which traps EryA in cellDepositorInsertmphA
UseTagsExpressionMutationPromoterPmphAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM98
Plasmid#220831PurposeEryA sensor with further increased sensitivity by mutation of mphR (T17R), in addition to mphA phosphorylationDepositorInsertmphR
UseTagsExpressionMutationT17RPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKPcR5
Plasmid#149467PurposeBroad-Host range expression vector, RK2 and pUC origin, PcaU(AM) repressor, P3B5B promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertPcaU(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP3B5BAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKVR5
Plasmid#149469PurposeBroad-Host range expression vector, RK2 and pUC origin, VanR(AM) repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertVanR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPVanCCAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSEVA3b61
Plasmid#58310PurposeShuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-tetR
Plasmid#62561PurposeTetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBLO44.1_dProtease
Plasmid#121631PurposeCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1DepositorInsertTEV protease
UseTagsExpressionMammalianMutationPromoterU6 and chicken beta-actin promoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-araC
Plasmid#62563PurposeArabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pJKR-H-mphR
Plasmid#62559PurposeMacrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
SUMO_TwinStrep_Csx30
Plasmid#197611PurposeFor protein purification of Csx30DepositorInsertSUMO_TwinStrep_Csx30
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM5411
Plasmid#132663PurposeRSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
UseTagsExpressionBacterialMutationPromoterPaadA and PconIIAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
EZ-L560
Plasmid#173028PurposeEncodes OptoAMP2 circuitDepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-acuR
Plasmid#62567PurposeAcrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
EZ-L545
Plasmid#173024PurposeEncodes OptoEXP2 circuitDepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-ttgR
Plasmid#62565PurposeFlavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.DepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
EZ-L580
Plasmid#173023PurposeEncodes OptoAMP4 circuitDepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationA79QPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only