Addgene

We narrowed to 3,013 results for: actin

Showing: 1001 - 1020 of 3013 results

Results per page:

Sort results by:

pTIGER_mNeonGreen::3xFLAG::dCrk

Plasmid

#131137

Purpose

UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Use

Tags

3XFLAG and mNeonGreen

Expression

Insect

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

Plasmid

#193717

Purpose

Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Synthetic, Human)

Use

Tags

2xmNeongreen-FRB

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-hsKIF5B(1-560)Rigor-L-mVenus

Plasmid

#193720

Purpose

Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Synthetic, Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Nup98-pDONR221

Plasmid

#124241

Purpose

Gateway entry clone of Drosophila Nup98-96 cDNA.

Depositor

Peter Vilmos

Article

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Nup98-96 (Nup98-96 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-Ctdnep1_Cter

Plasmid

#196522

Purpose

Entry vector for Gateway with Ctdnep1_Cter

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1-Cter (CTDNEP1 Human)

Use

Tags

Expression

Bacterial

Mutation

No transmembrane domain

Promoter

CMV promoter; T7 promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR201-Ctdnep1

Plasmid

#196520

Purpose

Entry vector for Gateway with Ctdnep1

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1-N1 (CTDNEP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

CMV promoter; T7 promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Ctdnep1_D67E-2xFlag

Plasmid

#196519

Purpose

Expression of Ctdnep1_D67E-2xFlag

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1_D67E-2xFlag (CTDNEP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

D67E mutation (phosphastase dead)

Promoter

CMV promoter; T7 promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1+CeGFP-Ctdnep1WTsiR

Plasmid

#196517

Purpose

Expression of Ctdnep1WT-GFP

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1WTsiR (CTDNEP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

CMV promoter; T7 promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 Myc-tagged PSK

Plasmid

#197149

Purpose

Expression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cells

Depositor

Jonathan Morris

Article

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Insert

TAOK2 (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
human TLNRD1-F250D pET151

Plasmid

#159385

Purpose

Expresses human TLNRD1 F250D mutant in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Changed Phenylalanine 250 to Aspartic Acid (F250D)

Promoter

T7

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-CLCb (EED/QQN)

Plasmid

#47422

Depositor

Peter McPherson

Article

Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

Insert

CLCb EED/QQN (CLTB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EED changed to QQN, in aa20-41 region

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pENTR CMVie-Lifeact-iRFP670 L1-R5

Plasmid

#84390

Purpose

Multisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Gateway

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-BASIC- ARHGAP29 Mutant PROMOTER

Plasmid

#104156

Purpose

luciferase reporter with ARHGAP29 mutant promoter

Depositor

Marius Sudol

Article

Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

Insert

ARHGAP29 promoter (ARHGAP29 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

delete two TEAD binding sites

Promoter

ARHGAP29

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR201-Eps8L2_494-546

Plasmid

#196531

Purpose

Entry vector for Gateway with Eps8L2_494-546

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_494-546 (EPS8L2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

CMV promoter; T7 promoter

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR201-Eps8L2_367-496

Plasmid

#196530

Purpose

Entry vector for Gateway with Eps8L2_367-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_367-496 (EPS8L2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

CMV promoter; T7 promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR201-Eps8L2_1-183

Plasmid

#196527

Purpose

Entry vector for Gateway with Eps8L2_1-183

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_1-183 (EPS8L2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

CMV promoter; T7 promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR201-Eps8L2_544-715

Plasmid

#196532

Purpose

Entry vector for Gateway with Eps8L2_544-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_544-715 (EPS8L2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

CMV promoter; T7 promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR201-Eps8L2_181-299

Plasmid

#196528

Purpose

Entry vector for Gateway with Eps8L2_181-299

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_181-299 (EPS8L2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

CMV promoter; T7 promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-Ctdnep1_C-ter

Plasmid

#196525

Purpose

Expression of Ctdnep1_C-ter

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1-C-ter (CTDNEP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

No transmembrane domain

Promoter

T7 promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR201-Eps8L2_297-370

Plasmid

#196529

Purpose

Entry vector for Gateway with Eps8L2_297-370

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_297-370 (EPS8L2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge