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Plasmid#105499PurposeExpression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)DepositorInsertISWI-iCBD
UseTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-2xTS
Plasmid#109419PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-APEX2
Plasmid#109420PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-26
Plasmid#172751PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-26
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cAbl
Plasmid#214234PurposeBacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorInsertABL1 Kinase domain (ABL1 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
UseTagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-21
Plasmid#172749PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-21
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-37
Plasmid#172756PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-37
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FL MALT1 WT in pET29b-Strep
Plasmid#48968PurposeExpresses Full length wild type MALT1 in e.coliDepositorInsertMALT1 isoform A (MALT1 Human)
UseTagsHis and StrepExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-SpyTag003
Plasmid#216282PurposeExpresses SUMO with a C-terminal SpyTag003 in bacterial cellsDepositorInsertSUMO-SpyTag003
UseTagsHis6 and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-rrt-His8
Plasmid#194088PurposeExpresses Ec86 reverse transcriptase in Escherichia coliDepositorInsertEc86 rrt
UseTagsHis8ExpressionMutationPromoterT7Available sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-GFP-mHP1alpha
Plasmid#181912PurposeBacterial protein expression vector for GFP-tagged HP1alphaDepositorInsertmHP1alpha (Cbx5 Mouse)
UseTagsGFPExpressionBacterialMutationPromoterT7Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-Mff(1-61)-PP-GST
Plasmid#73042PurposeBacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage siteDepositorInsertMff(1-61) (Mff Mouse)
UseTagsGST-tag and PreScission protease siteExpressionBacterialMutationTruncation containing amino acids 1-61PromoterT7Available sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-trAtCCD8(pYL735)
Plasmid#178286PurposeExpresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from ArabidopsisDepositorInserttrAtCCD8
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-EGFP
Plasmid#182641PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
UseTagsBAP, EGFP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LgBiT-SpyCatcher
Plasmid#223634PurposeProtein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis6, LgBiTExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26-MBP-GLIC
Plasmid#20887DepositorInsertpentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421
UseTagsHis, MBP, and pelBExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TEX264_wt-His6
Plasmid#220214PurposeBacterial expression of wild type TEX264, C-terminal His6 tagDepositorInsertTEX264 (TEX264 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21(+)-His6-Ubc13
Plasmid#212715PurposeBacterial expression of His6-Ubc13DepositorInsertUbc13
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA
Plasmid#99875PurposeExpression of GFP-AURKA for protein purification in bacteriaDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only