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p189
Plasmid#65207PurposeMutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.DepositorInsertEGFP
UseTagsExpressionBacterial and MammalianMutationPremature stop codon added to pGEM5Z(+)-EGFP (TGG…PromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCB-2
Plasmid#210388PurposeAll-in-one cumate-based inducible CRISPRi plasmid for use in StreptomycesDepositorInsertsdCas9
CymR
sgRNA cassette
UseCRISPR; Integrative vector for use in streptomycesTagsExpressionMutationBsaI-flanked RFP cassette inserted in place of sp…PromoterSP11 and SP30Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMEH31 Lamp2b HA
Plasmid#168208PurposeMammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tagDepositorInsertLamp2b (LAMP2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCB-1
Plasmid#210387PurposeAll-in-one cumate-based inducible CRISPRi plasmid for use in StreptomycesDepositorInsertsdCas9
CymR
sgRNA cassette
UseCRISPR; Integrative vector for use in streptomycesTagsExpressionMutationBsaI-flanked RFP cassette inserted in place of sp…PromoterSP11 and SP30Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCINeoFlag ALIX; I318A
Plasmid#89867PurposeExpresses full length Flag-tagged ALIX with mutation I318A in mammalian cells; internal ID WISP11-403DepositorInsertALIX (PDCD6IP Human)
UseTagsFlagExpressionMammalianMutationMutated isoleucine 318 to alaninePromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody
Plasmid#138132PurposeExpresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertEGFP-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-JNKAR
Plasmid#181857PurposeGenetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-JNKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-AktAR
Plasmid#181856PurposeGenetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AktAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIG PIK3CA E545K
Plasmid#73055PurposeHuman PIK3CA E545KDepositorInsertPIK3CA (PIK3CA Human)
UseTagsExpressionMammalianMutationE545KPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG PIK3CA Wildtype
Plasmid#73056PurposeHuman PIK3CA WildtypeDepositorInsertPIK3CA Wildtype (PIK3CA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody
Plasmid#138128PurposeExpresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmClover3-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody
Plasmid#138130PurposeExpresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmCherry-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 IRF9-STAT2C
Plasmid#37544DepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV3FLAG_hGTPBP4
Plasmid#53974PurposeMammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.DepositorInsertGTPBP4 (GTPBP4 Human)
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-Adrm1
Plasmid#19417DepositorInsertadhesion regulating molecule 1 (ADRM1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRb1/Cre
Plasmid#89647PurposeExpresses an Rb1-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Rb1 (Rb1 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB2 CTF-FLAG
Plasmid#217682PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB2 (BAI2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-911PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBSSK p85 alpha (delta iSH2)
Plasmid#13432DepositorInsertp85 alpha (delta iSH2) (PIK3R1 Human)
UseTagsflagExpressionBacterialMutationDominant negative p85 alpha. The p110 alpha bindi…PromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
Human HMG-CoA lyase like 1 (HMGCLL1)
Plasmid#52434Purposemammalian expression of human HMG-CoA lyase like 1 (HMGCLL1)DepositorInsertHMGCLL1 (HMGCLL1 Human)
UseTagsHISExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only
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