Addgene

We narrowed to 35,249 results for: lat

Showing: 10161 - 10180 of 35249 results

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pBAD/HisB (TIRSTD)-sfGFP

Plasmid

#189674

Purpose

titratable expression of sfGFP in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

sfGFP

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pmIL-6 -231

Plasmid

#61287

Purpose

drives luciferase via mouse IL-6 promoter, truncated (-231)

Depositor

Gail Bishop

Article

Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

Insert

Truncated IL-6 promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Deletion of 1046 nucleotides from the 5' end…

Promoter

IL-6 promoter (truncated)

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only
pMINTNH3

Plasmid

#110074

Purpose

Integrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage site

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Tags

His10 Tag - 3C cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1703

Plasmid

#78196

Purpose

optogenetic activation of IL2 neuron in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

ChR2(gf)

Use

Tags

Expression

Worm

Mutation

Promoter

klp-6

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pH6HTC_PEBP1-Halo

Plasmid

#175333

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PEBP1 (PEBP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDV05.2_pEF1a-iRFP720_secreted

Plasmid

#200409

Purpose

Expresses a secreted version of iRFP720 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720 (secreted)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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MSCB-hDot1Lmut

Plasmid

#74174

Purpose

For packaging hDot1Lmut (catalytic activity dead) to retrovirus.

Depositor

Yi Zhang

Article

Okada et al Cell. 2005 Apr 22;121(2):167-78.

Insert

hDot1L (DOT1L Human)

Use

Retroviral

Tags

HA

Expression

Mutation

hDot1L 163 GSG to RCR mutation*

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only
pLminP_Luc2P_RE43

Plasmid

#90381

Purpose

RUNX - Runx1 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

GATA3

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-13

Plasmid

#119992

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry

Use

Pcr template

Tags

mCherry

Expression

Mutation

Promoter

None

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-cueR_O

Plasmid

#127026

Purpose

Basic Part O- ORF; cueR; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part cueR_o

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pG108 - K

Plasmid

#178039

Purpose

Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

Depositor

Janina Lewis

Article

Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

Insert

Kanamycin resistance

Use

Tags

Expression

Bacterial

Mutation

ErmAM has been replaced with Kanamycin for select…

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-LMO-IRES-EGFP

Plasmid

#178309

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEAK10-His-Myc-Kal12

Plasmid

#25442

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal12

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pMEH381 VSV-G MS2 HA

Plasmid

#168223

Purpose

Mammalian expression of vesicular stomatitis virus glycoprotein fused to the MS2 dimer and C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

VSV-G

Use

Tags

HA and MS2 dimer

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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hSDH5

Plasmid

#113046

Purpose

Expression of WT hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5 (SDHAF2 Human)

Use

Tags

Expression

Bacterial

Mutation

Insert begins at amino acid 39

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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TOPO mCherry

Plasmid

#68490

Purpose

TOPO construct expressing mCherry for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mCherry

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-CreiLOV

Plasmid

#192817

Purpose

Expresses CreiLOV in bacteria

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

CreiLOV

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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human p53-(1-320)

Plasmid

#24864

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-320

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
p3E-U6a

Plasmid

#107593

Purpose

An entry vector with U6a promoter driving guide RNA expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

U6a promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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R26TV CAG LSL 2-5

Plasmid

#68412

Purpose

Rosa26 targeting vector backbone for use with GMAP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting; Gmap

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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