Addgene

We narrowed to 11,165 results for: cel.2

Showing: 10141 - 10160 of 11165 results

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psiCHECK2-AURKB 3'UTR

Plasmid

#29475

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

AURKB 3"UTR (AURKB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

Plasmid

#204214

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Deletion of DNA binding domain (delDBD)

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-flag-YTHDF2

Plasmid

#52300

Purpose

full length YTHDF2 with N-terminal flag tag for mammalian cell expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

T7

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pET15b-mLin28A-cHIS

Plasmid

#137998

Purpose

Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cells

Depositor

Judy Lieberman

Article

O'Day et al J Biol Chem. 2015 Jul 17;290(29):17909-2

Insert

mouse Lin28A, CSD and ZnFs (residues 36–186)

Use

Tags

C-terminal His6 tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCSC-NGN2-IRES-ISL1-T2A-LHX3

Plasmid

#221814

Purpose

Lentiviral vector expressing human NEUROG2, ISL1 and LHX3 for inducing pluripotent stem cells into cholinergic motor neurons.

Depositor

Baojin Ding

Article

Sepehrimanesh et al Am J Physiol Cell Physiol. 2020 Oct 1;31

Inserts

NEUROG2 (NEUROG2 Human)
ISL1 (ISL1 Human)
LHX3 (LHX3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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CapChR2_pmScarlet-N1

Plasmid

#188032

Purpose

Expresses CapChR2-mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-mScarlet

Use

Tags

mScarlet

Expression

Mammalian

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-synPCB2.1

Plasmid

#139478

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_053

Plasmid

#113591

Purpose

Constitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cells

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Commun. 2019 Apr 10;10(1):1668. doi:

Insert

sgRNA cloning site

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-OLIG2

Plasmid

#134668

Purpose

Retroviral expression of human OLIG2

Depositor

Marius Wernig

Article

Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

Insert

OLIG2 (OLIG2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

Plasmid

#120555

Purpose

Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

Depositor

Yu Wang

Article

Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

Insert

Cas9, NES, ERT2 and GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNAHygro(+)LAMP2B

Plasmid

#86029

Purpose

Expresses LAMP2B in Mammalian cells

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

Lysosome-associated membrane protein 2b (LAMP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry N1 TOMM22

Plasmid

#157762

Purpose

Expression of TOMM22:mcherry in cells

Depositor

Marc Tramier

Article

Bertolin et al Elife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

TOMM22 (TOMM22 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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MoFGFR2c-ires neo

Plasmid

#86492

Purpose

mamalian cell expression of FGFR2c

Depositor

David Ornitz

Article

Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

Insert

FgfrR2 IIIc 15D6 (Fgfr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Moloney murine leukemia virus LTR

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-synPCB2.0

Plasmid

#139477

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Use

Tags

Expression

Mammalian

Mutation

V413L

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM1-C49,50,83,103A

Plasmid

#58421

Purpose

Expresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteines 49, 50, 83, and 103 to Alanine

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pIK137

Plasmid

#65632

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-ARMS1-ProLink2-MRGPRX3

Plasmid

#213962

Purpose

Expresses MRGPRX3 in mammalian cells

Depositor

Christa Müller

Article

Al Hamwi et al Commun Biol. 2024 Jan 6;7(1):52. doi: 10

Insert

MRGPRX3 (MRGPRX3 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM1-C49,50,83A

Plasmid

#58420

Purpose

Expresses murine IFITM1-C49,50,83A with an N-terminal HA tag in mammalian cells. (mutated at the 3 evolutionarily conserved cysteines)

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteines 49, 50, and 83 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-N2A

Plasmid

#58410

Purpose

Expresses murine IFITM3-N2A with an N-terminal HA tag in mammalian cells (mutates possible glycosylation site)

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Asparagine 2 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008

Plasmid

#187916

Purpose

Expresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 2

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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