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pTETDuet_M42FG121VDHFR_R127ATS

Plasmid

#191305

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

M42F/G121V DHFR, R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTETDuet_D27NDHFR_WTTS

Plasmid

#191283

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

D27N DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTETDuet_M42FDHFR_WTTS

Plasmid

#191284

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

M42F DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_G121VDHFR_WTTS

Plasmid

#191285

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

G121V DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FG121VDHFR_WTTS

Plasmid

#191286

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

M42F/G121V DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_F31YG121VDHFR_WTTS

Plasmid

#191287

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

F31Y/G121V DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_WTDHFR_Q33STS

Plasmid

#191288

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

WT DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FDHFR_Q33STS

Plasmid

#191290

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

M42F DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_G121VDHFR_Q33STS

Plasmid

#191291

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

G121V DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ISA431: aTc-MP6

Plasmid

#189935

Purpose

Mutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanR

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

danQ926, dam, seqA, emrR, ugi and cda1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-CC-Di-C-Venus

Plasmid

#190710

Purpose

Encodes the parallel coiled-coil CC-Di followed by C-Venus.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

CC-Di-C-Venus

Use

Tags

Expression

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT SMASh

Plasmid

#182523

Purpose

C-SWAT compatible vector for C-terminal tagging with SMASh Degron. (Protease Cleavage Site: DEMEECSKHL).

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

SMASh Tag

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRPS5A-PTP-3Xflag- psa A Left -G1397N

Plasmid

#189645

Purpose

psa A Left -G1397N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AatII-pRPS5A-PTP-3Xflag- psa A Right _G1397N

Plasmid

#189647

Purpose

psa A Right _G1397N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 N

Plasmid

#189651

Purpose

rbc L 294 Right_G1397 N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRPS5A-PTP-3Xflag-16S rRNA Left_G1397N

Plasmid

#189641

Purpose

16S rRNA Left_G1397N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB174

Plasmid

#101673

Purpose

Opto-T7RNAP*(179)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(179)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB144

Plasmid

#101665

Purpose

Opto-T7RNAP*(69)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB153

Plasmid

#101664

Purpose

Opto-T7RNAP*(600)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(600)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Grp

Plasmid

#184292

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grp

Depositor

Bernardo Sabatini

Insert

sgRNA-Grp

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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