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Plasmid#174535PurposePUb-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsertPUb-mTurquoise BsaI cassette
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- PUb-DsRedSV40 GGGG AAGA
Plasmid#174538PurposePUb-DsRed fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsertPUb-DsRed BsaI cassette
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pProkR2.Cre.T2A.Venus
Plasmid#169014PurposeExpresses Cre under Prok receptor 2 minimal promoter in mammalian cellsDepositorInsertCre Recombinase
UseAAV and Cre/LoxTagsT2A and VenusExpressionMammalianMutationPromoterProkineticin Receptor 2Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-CRE-T2A-BleoR
Plasmid#172300PurposeIn vitro transcription of CRE-T2A-BleoR synthetic construct for the excision of floxed cassettesDepositorInsertCRE-T2A-BleoR
UseCre/LoxTagsExpressionMutationPromoterT7 promoter A-initiatingAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-CMV-GFP-miRE_shRen-Puro
Plasmid#135685PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup133_mEGFP(-9b)
Plasmid#163420PurposeMammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)DepositorInsertNup133 (NUP133 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup133_mEGFP(-8b)
Plasmid#163418PurposeMammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)DepositorInsertNup133 (NUP133 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZNF302-donor
Plasmid#132513PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF302 (ZNF302 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
TP2_crtYB
Plasmid#140233PurposeExpression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopeneDepositorInsertphytoene-beta carotene synthase
UseTagsExpressionBacterialMutationnaPromotert7 promoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTSHZ1-donor
Plasmid#132479PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertTSHZ1 (TSHZ1 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pPATZ1-donor
Plasmid#132522PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertPATZ1 (PATZ1 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
T2m
Plasmid#121029PurposeMoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertECK120033736 (164.6x Voigt terminator), short attachment
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7m
Plasmid#121030PurposeMoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertS. pyogenes tracrRNA terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T13m
Plasmid#121032PurposeMoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertECK120033737 (second strongest natural Voigt terminator)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C105m
Plasmid#121008PurposeMoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-AAV ssrA (has bbsI)
UseSynthetic BiologyTagsAAV degradation tag for ssrAExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C106m
Plasmid#121009PurposeMoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-DAS ssrA (has bbsI)
UseSynthetic BiologyTagsDAS degradation tag for ssrAExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only