Addgene

238,530 results

Showing: 10081 - 10100 of 238530 results

Results per page:

Sort results by:

pMBP-HIS2-Vps2

Plasmid

#21494

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

VPS2 (DID4 Budding Yeast)

Use

Tags

6x His and MBP

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pLV-EF1A-CD19.8H.8TM.BB.3z

Plasmid

#200671

Purpose

Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD8TM-41BB-CD3z-P2A-EGFP (CD8A, TNFRSF9, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAXY0001

Plasmid

#179834

Purpose

binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

Depositor

Xiaohan Yang

Article

Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

Insert

eYGFPuv

Use

Binary vector for plant transformation

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pIREShygro HA-mVenus-mAID

Plasmid

#117714

Purpose

Expression of HA-tagged mVenus fused to mAID

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mVenus-mAID

Use

Tags

triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pIREShygro HA-mVenus-mAID

Plasmid

#117714

Purpose

Expression of HA-tagged mVenus fused to mAID

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mVenus-mAID

Use

Tags

triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLifeAct_mScarlet-H_N1

Plasmid

#85055

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLifeAct_mScarlet-H_N1

Plasmid

#85055

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBtet-GH

Plasmid

#60498

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

TCE & RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBtet-GH

Plasmid

#60498

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

TCE & RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pG12-SEAP

Plasmid

#170271

Purpose

Secreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

SEAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

12xUAS with TATA-box minimal promoter

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Kv2.1/RBG4

Plasmid

#196947

Purpose

Plasmid expressing an antigen targeting Kv2.1 (RBG4)

Depositor

James Trimmer

Insert

Kv2.1 (Kcnb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7C-DNAJB6

Plasmid

#177657

Purpose

Recombinant protein synthesis of human DNAJB6

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

DNAJB6 (DNAJB6 Human)

Use

Tags

6xHis-Thrombin

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Glycogene CRISPR Library

Pooled Library

#140961

Purpose

Targets human genes involved in cellular glycosylation.

Depositor

Sriram Neelamegham

Article

Zhu et al Glycobiology. 2020 Aug 8. pii: 5889976.

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only
pDest-795

Plasmid

#161881

Purpose

Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Type

Empty backbone

Use

Tags

MBP

Expression

Insect

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-TetO-hNGN2-Neo

Plasmid

#99378

Purpose

Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

hNGN2-P2A-Neo (NEUROG2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-TetO-hNGN2-Neo

Plasmid

#99378

Purpose

Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

hNGN2-P2A-Neo (NEUROG2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW016-2

Plasmid

#85613

Purpose

Cas9-tracrRNA integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

cas9-tracrRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW016-2

Plasmid

#85613

Purpose

Cas9-tracrRNA integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

cas9-tracrRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
His-RIAM

Plasmid

#32803

Depositor

Vicki Boussiotis

Insert

RIAM (APBB1IP Human)

Use

Tags

His and XPress

Expression

Mammalian

Mutation

Promoter

T7

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
His-RIAM

Plasmid

#32803

Depositor

Vicki Boussiotis

Insert

RIAM (APBB1IP Human)

Use

Tags

His and XPress

Expression

Mammalian

Mutation

Promoter

T7

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only