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Plasmid#201081PurposeIm7 WT inserted into CysGA at G364 via GS linkerDepositorInsertCysGA-Im7
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrc-CysGA-Linker only
Plasmid#201080PurposeGS linker inserted into CysGA at G364DepositorInsertCysGA-Linker only
UseTagsExpressionBacterialMutationPromoterpTrcAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pML31
Plasmid#203343Purpose3 input AND circuit on pLW555 backbone: PTet-P1, PLux-A1, PIcaRA:PPhlF:PAmtR-Q2, PTac-I1DepositorInsertsphlF
amtR
qacR
icaRA
UseTagsExpressionMutationPromoterPLux, PPhlF, PIcaRA, and PAmtR, PTac, and PTetAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_Q33STS
Plasmid#191289PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationD27N DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_Q33STS
Plasmid#191292PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R166QTS
Plasmid#191311PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_WTTS
Plasmid#191310PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_Q33STS
Plasmid#191313PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_Q33STS
Plasmid#191293PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y/G121V DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_R166QTS
Plasmid#191294PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationWT DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_R166QTS
Plasmid#191295PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationD27N DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FDHFR_R166QTS
Plasmid#191296PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_G121VDHFR_R166QTS
Plasmid#191297PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationG121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_R166QTS
Plasmid#191298PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_R166QTS
Plasmid#191299PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y/G121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_WTDHFR_R127ATS
Plasmid#191300PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationWT DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_R127ATS
Plasmid#191301PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationD27N DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FDHFR_R127ATS
Plasmid#191302PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_G121VDHFR_R127ATS
Plasmid#191303PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationG121V DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YG121VDHFR_R127ATS
Plasmid#191304PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y/G121V DHFR, R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only